SAKURAI Akihiko

FacultyApplied Chemistry and Biotechnology
Teacher OrganizationApplied Chemistry and Biotechnology
Education and
 Research Organization
Faculty of Engineering /Graduate School of Engineering
PositionProfessor
Last Updated: 19/10/15 21:46

Researcher Profile & Settings

Name

    SAKURAI Akihiko

Research Areas

  • Engineering / Process/Chemical engineering / Production and characterization of bioactive substances, Development of bioprocess using enzymes and/or microorganismsEnzyme, Bioactive substances, Bioreactor, Environmental pollution

Affiliation

  •  Applied Chemistry and Biotechnology Professor

Education

  • Mar. 1992Hokkaido University 合成化学工学
  • Apr. 1983Mar. 1987Hokkaido University Department of Chemical Process Engineering

Academic & Professional Experience

  • Apr. 1992Mar. 19961992-1996 Japan Synthetic Rubber Co. Ltd. Researcher

Overseas travel history

  • カナダ McGill University Sep.  2006- Dec. 2006

Research Activities

Published Papers

  • Simple and efficient isolation of cordycepin from culture broth of a Cordyceps militaris mutant
    M. Masuda, M. Hatashita, S. Fujihara, Y. Suzuki, A. Sakurai
    J. Biosci. Bioeng 120 732-735 2015 Refereed
  • Efficient production of cordycepin by the Cordyceps militaris mutant G81-3 for practical use
    Masuda, Mina;Das, Shonkor Kumar;Hatashita, Masanori;Fujihara, Shinya;Sakurai, Akihiko
    PROCESS BIOCHEMISTRY 49(2) 181-187 2014 Refereed
  • Cordycepin production by a repeated batch operation of RDC using a Cordyceps militaris mutant
    A.Sakurai, M. Masuda, M. Hatashita
    Proceedings of Asian Congress on Biotechnology 2013 Dec.  2013
  • Formate Oxidase, an Enzyme of the Glucose-Methanol-Choline Oxidoreductase Family, Has a His-Arg Pair and 8-Formyl-FAD at the Catalytic Site
    D.Doubayashi, T.Ootake, Y.Maeda, M.Oki, Y.Tokunaga, A.Sakurai, Y.Nagaosa, B.Mikami, H.Uchida
     75 1662-1667 Sep.  2011 Refereed
  • Analysis of extracellular protein during manganese peroxidase production by white rot fungus
    S. Fujihara, Y. Koketsu, M. Hatashita, A.Sakurai
     A1432 May  2011
  • Effect of light on cultured mycelia and cordycepin production using Cordyceps militaris in surface liquid culture
    S. K. Das, S. Fujihara, M. Masuda, A.Sakurai
     159 Mar.  2011
  • Production of cordycepin by repeated batch culture of Cordyceps militaris mutant obtained by proton beam irradiation
    M. Masuda, S. K. Das, S. Fujihara, M. Hatashita, A.Sakurai
     111 55-60 Jan.  2011 Refereed
  • Production of manganese peroxidase by Bjerkandera sp. mutant induced by ion beam irradiation
    A.Sakurai, Y. Koketsu, S.Fujihara, M. Hatashita
     P-I.80 Sep.  2010
  • Medicinal uses of the mushroom cordyceps militaris: current state and prospects
    S. K. Das, M. Masuda, S. Fujihara, A.Sakurai
     81 961-968 Jun.  2010 Refereed
  • Production of manganese peroxidase by white rot fungi from potato-processing wastewater: Role of amino acids on biosynthesis
    S. Fujihara, M. Hatashita, A.Sakurai, M.Sakakibara
     9 725-731 Jan.  2010 Refereed
  • Efficient Production of Anticancer Agent Cordycepin by Repeated Batch Culture of Cordyceps militaris Mutant
    S. K. Das, S. Fujihara, A.Sakurai, M. Masuda
     654-660 Jan.  2010
  • Optimization of culture medium for cordycepin production using Cordyceps militaris mutant obtained by ion beam irradiation
    S.K.Das, M.Masuda, M.Hatashita, A.Sakurai, M.Sakakibara
     45 129-132 2010 Refereed
  • Expression in Escherichia coli of an unnamed protein gene from Aspergillus oryzae RIB40 and cofactor analyses of the gene product as formate oxidase
    Y.Maeda, D.Doubayashi, M.Oki, H.Nose, A.Sakurai, K.Isa, Y.Fujii, H.Uchida
    Biosci.Biotechnol.Biochem. 73 2645-2649 Dec.  2009 Refereed
  • Performance of Cordyceps militaris mutant regarding anti-cancer agent cordycepin production in submerged culture obtained by ion beam irradiation
    S. K. Das, M. Masuda, M. Hatashita, A.Sakurai, M.Sakakibara
     7 42013 Dec.  2009 Refereed
  • Expression analysis of manganese peroxidase gene in white rot fungus L-25 in the presence of amino acids
    S.Fujihara, A.Sakurai, M.Sakakibara
     FM-P22 Nov.  2009
  • Structural features and immuno-stimulating effect of the polysaccharide fractions from Cordyceps militaris
    M.Masuda, A.Sakurai, M.Sakakibara
     FE-P2 Nov.  2009
  • Effect of additives on cordycepin production using the Cordyceps militaris mutant induced by ion beam irradiation
    S.K.Das, M.Masuda, A.Sakurai, M.Sakakibara
     9060172 Sep.  2009
  • Nonylphenol removal using Coprinus cinereus peroxidase
    H. R. Kariminiaa, A.Sakurai, M.Sakakibara
     285 Aug.  2009
  • Effect of Amino Acids on Manganese Peroxidase Production
    S. Fujihara, A.Sakurai, M.Sakakibara
     1156 Aug.  2009
  • Frontier prospects of medicinal mushroom Cordyceps militaris in the realm of cancer research
    S.K.Das, M.Masuda, A.Sakurai, M.Sakakibara
     504 Jun.  2009
  • Effect of additives on cordycepin production using a Cordyceps militaris mutant induced by ion beam irradiation
    S.K.Das, M.Masuda, A.Sakurai, M.Sakakibara
     8(13) 3041-3047 Jan.  2009 Refereed
  • A new approach for improving cordycepin productivity in surface liquid culture of Cordyceps militaris using high-energy ion beam irradiation
    S.K.Das, M.Masuda, M.Hatashita, A.Sakurai, M.Sakakibara
     47 534-538 Dec.  2008 Refereed
  • A newly isolated white-rot fungus capable of producing high levels of manganese peroxidase
    Hamid Reza Kariminia, A.Sakurai, M.Sakakibara
     P94 Sep.  2008 Refereed
  • Nonylphenol removal using Coprinus cinereus peroxidase
    H. R. Kariminia, A.Sakurai, M.Sakakibara
     Aug.  2008 Refereed
  • Optimization of medium components for manganese peroxidase production by white rot fungus strain L-25
    S. Fujihara, A.Sakurai, M.Sakakibara
     41 796-803 Jul.  2008 Refereed
  • Characterization of aldehyde oxidase from Brevibacillus sp. MEY43 and its application to oxidative removal of glutaraldehyde
    Y. Maeda, A. Yagyu, A.Sakurai, Y.Fujii, H.Uchida
     2008 Refereed
  • Degradation of bisphenol A using sonochemical reactions
    Marcio Inoue, Yukihiro Masuda, Fumio Okada, A.Sakurai, I.Takahashi, M.Sakakibara
     42 1379-1386 2008 Refereed
  • Development of a system to degrade bisphenol A using sonochemical reactions
    Marcio Inoue, Yukihiro Masuda, Fumio Okada, Akihiko Sakurai, Mikio Sakakibara
     P-39 Dec.  2007
  • Formaldehyde Degradation by A Newly Isolated Bacterium
    A.Sakurai, M.Sakakibara
     E0059 Nov.  2007 Refereed
  • Optimization of Medium Components for Manganese Peroxidase Production by White Rot Fungus Strain L-25
    S. Fujihara, A.Sakurai, M.Sakakibara
     G0058 Nov.  2007 Refereed
  • Decolorization of synthetic dyes by a new manganese peroxidase-producing white rot fungus
    H.-R. Kariminiaae-Hamedaani, A.Sakurai, M.Sakakibara
     72 157-162 2007 Refereed
  • Purification, characterization, and potential applications of formate oxidase from Devaryomyces vanrijiae MH201
    H.Uchida, M.Hojyo, Y.Fujii, Y.Maeda, R.Kajimura, H.Yamanaka, A.Sakurai, M.Sakakibara, K.Aisaka
     74 805-812 2007 Refereed
  • Enhanced production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris
    M.Masuda, E.Urabe, H.Honda, A.Sakurai, M.Sakakibara
     40 1199-1205 2007 Refereed
  • Degradation of hazardous phenols using sonochemical reactions
    M.Inoue, T.Kimura, F.Okada, A.Sakurai, M.Sakakibara
     Paper ID 70 Aug.  2006
  • Removal of xenobiotic compounds by a manganese peroxidase-producing white-rot fungus
    A.Sakurai, H.R.Kariminiaase-Hamedaani, M.Nakai, M.Sakakibara
     Paper ID 228 Aug.  2006
  • A new development of dyestuffs degradation system using ultrasound
    M.Inoue, F.Okada, A.Sakurai, M.Sakakibara
     13 313-320 2006 Refereed
  • Production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris
    M.Masuda, E.Urabe, A.Sakurai, M.Sakakibara
     39 641-646 2006 Refereed
  • Study towards cellular degradation caused by environmentally persistent chemical
    S.K.Das, M.Sakakibara, A.Sakurai, J.Bhattacharjee, M.M.Rahman, M.A.Awal
     6 1083-1087 2006 Refereed
  • Removal of dioxins, endocrine disruptors and dyes by a newly isolated white-rot fungus
    A.Sakurai, M.Nakai, H.Kariminiaae-Hamedaani, S.Fujihara, M.Sakakibara
     CD-ROM723 Dec.  2005
  • Production of Coprinus cinereus peroxidase and its use for the removal of alkylphenols
    A.Sakurai, S.Yokogawa, M.Sakakibara
     Jul.  2005 Refereed
  • Development of a new dyestuffs degradation system using ultrasound
    M.Inoue, F.Okada, A.Sakurai, M.Sakakibara
     1P-06-021 Jan.  2004 Refereed
  • Production of Coprinus cinereus peroxidase and its use for the removal of nonylphenol
    A.Sakurai, K.Fujita, J.F.Abarca, M.Sakakibara
     2J-10 Jan.  2004 Refereed
  • Removal of bisphenol A by peroxidase-catalyzed reaction using culture broth of Coprinus cinereus
    A.Sakurai, S.Toyoda, M.Masuda, M.Sakakibara
     37 137-142 2004 Refereed
  • Rapid pyrolysis of wood block by microwave heating
    M.Miura, H.Kaga, A.Sakurai, T.Kakuchi, K.Takahashi
     71  187-199 2004 Refereed
  • Effect of Surfactants on Phenol Removal by the Method of Polymerization and Precipitation Catalysed by Coprinus cinereus Peroxidase
    A.Sakurai, M.Masuda, M.Sakakibara
     2003 Refereed
  • Preparation and Properties of Transparent Cellulose Hydrogels
    H.Saito, A.Sakurai, M.Sakakibara, H.Saga
     90 3020-3025 2003 Refereed
  • Peroxidase production by Coprinus cinereus using rotating disk contactor
    A.Sakurai, S.Kawamoto, J.F.Abarca, M.Sakakibara
     2002 Refereed
  • Removal of lignin in liquid system by an isolated fungus
    A.Sakurai, T.Yamamoto, A.Makabe, S.Kinoshita, M.Sakakibara
     2002 Refereed
  • Effect of temperature and pH on phenol removal using purified Coprinus cinereus peroxidase
    M.Masuda, A.Sakurai, M.Sakakibara
     2002 Refereed
  • Removal of bisphenol A by peroxidase-catalyzed reaction using culture broth of Coprinus cinereus
    A.Sakurai, S.Toyoda, M.Masuda, M.Sakakibara
     2002 Refereed
  • Effect of reaction conditions on phenol removal by polymerization and precipitation using Coprinus cinereus peroxidase
    M.Masuda, A.Sakurai, M.Sakakibara
     2001 Refereed
  • Removal of bisphenol A by polymerization and precipitation method using Coprinus cinereus peroxidase
    A.Sakurai, S.Toyoda, M.Sakakibara
     2001 Refereed
  • Ethanol production from starch by immobilized Aspergillus awamori and Saccharomyces pastorianus using cellulose carriers
    N.Fujii, T.Oki, A.Sakurai, S.Suye, M.Sakakibara
     2001 Refereed
  • Effect of enzyme impurities on phenol removal by the method of polymerization and precipitation using Coprinus cinereus peroxidase
    M.Masuda, A.Sakurai, M.Sakakibara
     2001 Refereed
  • Effect of inducer addition on production of 4,5-dihydroxyphthalate by Pseudomonas testosteroni
    A.Sakurai, M.Sakakibara
     2000 Refereed
  • Ethanol production by repeated batch culture using yeast cells immobilized on porous cellulose carriers
    A.Sakurai, Y.Nishida, H.Saito, M.Sakakibara
     2000 Refereed
  • Influence of surface chatacteristics of cellulose carriers on ethanol production by immobilized yeast cells
    N.Fujii, A.Sakurai, K.Onjoh, M.Sakakibara
     1999 Refereed
  • Production of 4,5-dihydroxyphthalate by Pseudomonas testosteroni immobilized in alginate gel beads
    A.Sakurai, M.Sakakibara
     1999 Refereed
  • The use of biofilm dipping reactor for citric acid production
    A.Sakurai, H.Imai, M.Sakakibara
     1999 Not refereed
  • Decontamination of phenol by peroxidase-catalyzed polymerization
    A.Sakurai, S.Kawamoto, J.F.A.Montoya, M.Sakakibara
     1999
  • Simple and efficient isolation of cordycepin from culture broth of a Cordyceps militaris mutant(METHODS)
    Masuda Mina;Hatashita Masanori;Fujihara Shinya;Suzuki Yu;Sakurai Akihiko
    Journal of bioscience and bioengineering 120(6) 732-735 Dec.  2015
    Isolation of cordycepin from the culture broth of Cordyceps militaris mutant was investigated. Based on the solubility curve, three crystallizing processes, temperature shift (process I), pH shift (process II), and pH shift followed by temperature shift (process III) were carried out. Process III was the most promising method regarding both purity and yield.

Books etc

  • Recent research developments in biotechnology & bioengineering
    A.Sakurai, H.Imai, M.Sakakibara
     1999
  • Wastewater Treatment Using Enzymes
    A.Sakurai, M.Masuda, M.Sakakibara
     2003
  • Current Topics in Biotechnology, vol. 2
    A.Sakurai, M.Sakakibara
     2005

Conference Activities & Talks

  • Degradation of brominated flame retardant HBCD by ultrasonic irradiation
    Y. Wei, K. Shota, A.Sakurai
     Sep.  2013
  • Stenotrophomonas属微生物によるホルムアルデヒドの分解
    櫻井 明彦, 小越 多美, 水谷 日出夫, 河村 昂介, 榊原 三樹男
     Sep.  2007
  • Optimization of C & N sources for cordycepin production using promising Cordyceps militaris mutant obtained by proton beam irradiation
    Das Shonkor Kumar, Mina Masuda, Masanori Hatashita, A.Sakurai, M.Sakakibara
     Sep.  2007