SUYE Shin-ichiro

FacultyPresident / Vice-president
Teacher OrganizationPresident / Vice-president
Education and
 Research Organization
President / Vice-president
PositionTrustee, Vice President for Research, Industry-Academia Collaboration, and Social Cooperation
Last Updated: 19/07/05 18:44

Researcher Profile & Settings

Name

    SUYE Shin-ichiro

Affiliation

  •  President / Vice-president Trustee, Vice President for Research, Industry-Academia Collaboration, and Social Cooperation
  •  University Library
  •  Organization for Comprehensive Nuclear Research in Medical Science and Engineering
  •  Organization for Society-Academia Cooperation and Regional Innovation Promotion
  •  Center for Promotion of Gender Equality

Education

  • Apr. 1981Mar. 1983Tokyo University of Agriculture and Technology Agriculture 農芸化学
  • 1981Tokyo University of Agriculture and Technology

Academic & Professional Experience

  • Apr. 1983Mar. 1988Central Research Laboratory, Takara Shuzo Co., Research Associate

Research Activities

Published Papers

  • Electrochemical behavior of dye-linked L-proline dehydrogenase on glassy carbon electrode modified by multi-walled carbon nanotubes
    H. Zheng, Y. Okezaki, R. Kawakami, H. Sakuraba, T. Ohshima, S. Suye
    Beilstain J. Nanotechnol., 1 135-141 Dec.  2010 Refereed
  • Yeast co-displaying enhanced green fluorescent protein and organophosphorus hydrolase in combination with a cell chip for detection of organophosphorus compounds
    T. Fukuda, K. Tsuchiyama, H. Makishima, A. Mulchandani, K. Kuroda, M. Ueda, S. Suye
    Biotechnol. J., 5 515-519 Jul.  2010 Refereed
  • Demonstration of catalytic proton acceptor of chitosanase from Paenibacillus fukuinensis by comprehensive analysis of mutant library
    D. Isogawa,, T. Fukuda,, K. Kuroda,, H. Kusaoke,, H. Kimoto, S. Suye, M. Ueda
     85 95-104 Jun.  2009 Refereed
  • Construction of L-lysine sensor by layer-by-layer adsorption of L-lysine dehydrogenase and ferrocene-labeled high molecular weight coenzyme derivative on gold electrode
    H. Zheng, J. Zhou, J. Zhang, R. Huang,, H. Jia, Y. Okezaki, S. Suye
     20 2685-2691 Sep.  2008 Refereed
  • Oriented immobilization and electron transfer of hyperthermostable L-proline dehydrogenase on the modified electrode
    S. Suye, T. Terai, S. Shiraishi, H. Sakuraba, T. Ohshima
     May  2008
  • Organophosphorus compounds sensing system using organophosphorus hydrolase and EGFP displayed arming yeast
    H. Makiasima, T. Fukuda, K. Kuroda,, A. Mulchandani, K. Takayama, S. Suye, M. Ueda
     May  2008
  • Improvement in enzymatic desizing of starched cotton cloth using yeast co-displaying glucoamylase and cellulose-binding domain
    T.Fukuda, M.Kato-Murai,, K.Kuroda,, M.Ueda, S.Suye
     77 1225-1232 Mar.  2008 Refereed
  • Reverse Reaction of Malic Enzyme for HCO3- Fixation into Pyruvic
    Y.Ohno, T.Nakamori, H.Zheng, S.Suye
     72 70772-70775 Mar.  2008 Refereed
  • Enhancement of substrate recognition ability by combinatorial mutation of beta-glucosidase displayed on the yeast cell surface
    T.Fukuda, S.Suye, H.Sahara, M.Ueda
    Appl. Microb. Biotechnol. 76 1027-1033 Nov.  2007 Refereed
  • Sol?gel silica planar waveguide doped with green fluorescent protein for in-line biosensors
    Y.Enami, T.Fukuda, S.Suye
     91  203507-1-3 Nov.  2007 Refereed
  • Biodegradation of poly (?-caprolactone) film in presence of Lysinibacillus sp. 70038 and characterization of the degraded film
    S. Xu, T. Yamaguchi, S. Osawa, S.Suye
     12 119-122 Aug.  2007 Refereed
  • Development of high-throughput screening system by single cell reaction usingmicrochamber array chip
    T.Fukuda, M.Kato, S.Suye, M.Ueda
    J. Biosci. Bioengeer.. 104 241-243 Jan.  2007 Refereed
  • Yeast cell surface expression of chitosanase from Paenibacillus fukuinensis
    T.Fukuda,, D.Isogawa,, M.Takagi, M.Kato, H.Kimoto, H.Kusaoke, M.Ueda, S.Suye
     71 2845-2847 Jan.  2007 Refereed
  • Purification and Properties of Diethyl p-Phthalate Esterase from Ochrobactrum anthoropi 6-2b
    S.Xu, C.Komatsu, I.Takahashi, S.Suye
    Sen-I Gakaki Shi 62(10) 226-231 Jan.  2006 Refereed
  • Microbial Degradation of Ethylene Glycol Dibenzoate by Rhodococcus sp. 2Cb
    S.Xu, T.Yamaguchi, S.Suye, T.Hori
    Sen-I Gakaki Shi 62 106-110 2006 Refereed
  • Enhancement of cellulase activity by the clones selected from the combinatorial library of the cellulose-binding domain by cell surface engineering
    T.Fukuda, T.Ishikawa, M.Ogawa, S.Shiraga, M.Kato, S.Suye, M.Ueda
    Biotechnol. Prog. 22 933-938 2006 Refereed
  • Surface Display of Organophosphorus Hydrolase on Saccharomyces cerevisiae
    K. Takayama,, S.Suye, K.Kuroda, M.Ueda, T.Kitaguchi, K.Tsuchiama, T.Fukuda, W.Chen, A.Mulchandani
    Biotechnol. Prog. 22 939-943 2006 Refereed
  • Construction of cultivation system of a yeast single cell in a cell chip chamber
    T.Fukuda, S.Shiraga, M.Kato, S.Suye, M.Ueda
    Biotechnol. Prog. 22 944-948 2006 Refereed
  • L-Proline sensor based on layer-by-layer immobilization of thermostable dye-linked L-proline dehydrogenase and polymerized mediator
    H.Zheng, Y.Hirose, T.Kimura, T.Hori, S.Suye, H.Katayama, J.Arai, R.Kawakami, T.Ohshima
    Sci. Technol. Adv. Mat. 7 243?248 2006 Refereed
  • Detection System for Organic Vapors Using Cholesteric Liquid Crystal
    M.Tokuda, S.Suye, T.Hori, I.Imae, Y.Kawakami, H.Katayama
    he 2nd International Meeting on Microsensors and Microsystems, PC29 Tainan, Taiwan 2006
  • Construction of Recombinant Yeast Displayed Organophosphorus Hydrolase and Enhanced Green Fluorescent Protein on the Cell Surface for Detection of Organophosphorus Compounds
    K.Tsuchiyama, T.Kitaguchi, T.Fukuda, K.Kuroda, K.Takayama, A.Mulchandani, S.Suye, M. Ueda
    The 2nd International Meeting on Microsensors and Microsystems, PC25 Tainan, Taiwan 2006
  • Novel High-throughput Screening System of a Single Cell in Combination with Cell Surface Engineering and Chip Technology
    T.Fukuda, M.Kato, S.Suye, M.Ueda
    The 2nd International Meeting on Microsensors and Microsystems, S6 Tainan, Taiwan 2006
  • Layer-by-layer and Oriented Immobilization of and Electron Transfer of Hyperthermostable L-proline Dehydrogenase on the Modified Electrode
    S.Suye, Y.Hirose, M.Zaleska, H.Zheng, T.Hori, R.Kawakami, H.Sakuraba, T.Ohshima
    The 2nd International Meeting on Microsensors and Microsystems, F8 Tainan, Taiwan 2006
  • Oriented Immobilization and Electron Transfer of Hyperthermostable L-proline Dehydrogenase on the Modified Electrode
    Y.Hirose, M. Zaleska, S.Suye, T.Hori, R. Kawakami, H. Sakuraba, T. Ohshima
     21 Nov.  2005
  • Assembly of alternating polymerized mediator, polymerized coenzyme, and enzyme modified electrode by layer-by-layer adsorption technique
    S.Suye, H. Zheng, H. Okada, T.Hori
    (108) 671-675 2005 Refereed
  • A rapid and simple determination of food-borne Salmonella strains by using multi-channel oxygen electrode
    Y. Nambo, T. Matsuura, A. Murakami, S.Suye, T.Hori, K. Isshiki
     10 73-77 2005 Refereed
  • Amperometric DNA sensor using gold electrode modified with polymeric mediator by layer-by-layer adsorption
    S.Suye, T. Matsuura, T. Kimura, H. Zheng, T.Hori, Y. Amano, H. Katayama
    (81) 441-447 2005 Refereed
  • Construction of a novel single cell screening system by yeast cell chip
    T. Fukuda, S. Shiraga, M. Kato, S. Morita, E. Tamiya, T.Hori, S.Suye, M. Ueda
    (1) 105-112 2005 Refereed
  • Construction of a biocathode using the multicopper oxidase from the hyperthermophilic archaeon, Pyrobaculum aerophilum: towards a long-life biobattery
    Sakamoto, Hiroaki;Uchii, Toshiki;Yamaguchi, Kayo;Koto, Ayako;Takamura, Ei-ichiro;Satomura, Takenori;Sakuraba, Haruhiko;Ohshima, Toshihisa;Suye, Shin-ichiro
    BIOTECHNOLOGY LETTERS 37(7) 1399-1404 Nov.  2015 Refereed
  • Amperometric Determination of Ascorbic Acid on an Au Electrode Modified by a Composite Film of Poly(3,4-ethylenedioxythiophene) and Superconductive Carbon Black
    ZHOU Xinfei;HE Kunyun;WANG Yu;ZHENG Haitao;SUYE Shin-ichiro
    Analytical Sciences 31(5) 429-436 Nov.  2015 Refereed
    A new composite film-modified electrode was prepared by the electropolymerisation of poly(3,4-ethylenedioxythiophene) (PEDOT) and superconductive carbon black (SCB) on a gold electrode. The PEDOT-SCB/Au electrode exhibited excellent ability towards the electrocatalytic oxidation of ascorbic acid, in terms of a 480 mV shift of the oxidation potential in the negative direction, and a dramatically enhanced oxidation current. Under the optimum conditions, the amperometric detection of ascorbic acid provided a wide linear detection range from 1.0 × 10−7 to 8.0 × 10−4 M, and a detection limit of 5.0 × 10−8 M (S/N = 2) as well as good reproducibility and stability.
  • Estimation of the Core-Shell Formation Efficiency of Electrospun Collagen/Polylactic Acid Nanofibers
    KATO Arata;SUYE Shin-ichiro;FUJITA Satoshi
    KOBUNSHI RONBUNSHU 0 2016
    Nanofibers are expected to become useful as medical materials including tissue engineering scaffolds, wound dressings, and carriers for drug delivery systems (DDS), because of their high specific surface area, porosity, anisotropy and the molecular order. Especially, the potential of core-shell nanofibers, that are composed of two types of materials for core and shell, are drawing attention to DDS materials because it is possible to suppress the diffusion of the drug from the core material through the shell material to realize a more precise controlled release. To use core-shell fibers in DDSs, the detailed characterization of the structure, including core-shell ratio, the surface area, and the core-shell formation efficiency is required. However, the structural analysis of individual fibers has not yet been fully carried out, although bulk properties have been well examined. Here, to obtain key structural information for DDS materials, we estimated the efficiency of polymer incorporation into complete core-shell fibers from cross-sectional images of the core-shell nanofibers by SEM observation.
  • 3P-028 Construction of biocathode for biofuel cell using multicopper oxidase from hyperthermophilic archaeon, Pyrobaculum aerophilum
    Takamura Eiichiro;Uchii Toshiki;Sakamoto Hiroaki;Satomura Takenori;Sakuraba Haruhiko;Ohshima Toshihisa;Suye Shin-ichiro
     67 Sep.  2015
  • Construction of Biodevise with Created Biocatalyst for Biofuel Cells
    SUYE SHIN‐ICHIRO;SAKAMOTO HIROAKI;SATOMURA TAKENORI
    Sen'i Gakkaishi 72(4) P-209-P-210 2016
  • Observation of Electrospun Nanofiber Surface by Atomic Force Microscopy
    SAKAMOTO HIROAKI;SUYE SHIN‐ICHIRO
    Sen'i Gakkaishi 72(4) P-207-P-207 2016
  • Design of a multi-enzyme reaction on an electrode surface for an l-glutamate biofuel anode
    Sakamoto, Hiroaki;Komatsu, Tomohiro;Yamasaki, Koji;Satomura, Takenori;Suye, Shin-ichiro
    BIOTECHNOLOGY LETTERS 39(2) 235-240 Feb.  2017
  • Fabrication and Characterization of Fe/Polyurethane Nanofiber Actuator Prepared by Electrospinning
    Sakamoto, Hiroaki;Amaya, Satoshi;Sunahase, Yuki;Suye, Shin-ichiro
    JOURNAL OF FIBER SCIENCE AND TECHNOLOGY 73(6) 135-138 Jun.  2017
  • Evaluation of protein adsorption onto a polyurethane nanofiber surface having different segment distributions
    Morita, Yuko;Koizumi, Gaku;Sakamoto, Hiroaki;Suye, Shin-ichiro
    MATERIALS CHEMISTRY AND PHYSICS 187 1-4 Feb.  2017
  • 3P-029 Construction of bioanode using glutamate dehydrogenase from Pyrobaculum islandicum and polymerized coenzyme
    Yamazaki Koji;Sakamoto Hiroaki;Satomura Takenori;Sakuraba Haruhiko;Ohshima Toshihisa;Suye Shin-ichiro
     67 Sep.  2015
  • Design and Fabrication of Conductive Nanofiber Using Electrospinning
    Sakamoto Hiroaki;Fujita Satoshi;Suye Shin-ichiro
    IEEJ Transactions on Sensors and Micromachines 136(11) 461-464 2016

    Nanofibers have exceptional properties due to their diameter and large surface area to mass ratio. Thus, nanofibers can potentially be used as electrodes in highly sensitive biosensing systems, bio-fuel cells, and high-power devices. In this study, conductive polyurethane-FeCl3 composite nanofibers were fabricated by electrospinning. The optimal electrospinning conditions were investigated, and the physical and chemical properties of the composite were evaluated. Conductive polyurethane/FeCl3 fibers of 1-5 µm in diameter were obtained, and their conductivity was 13.7±1.47×10-3 S/m. The effects of the morphology of the material were also monitored, and it was found that a fiber mat was 10 times more conductive than a film, because the crystallinity of the polyurethane and the Fe3+ distribution affect the electrical properties. The conductive nanofibers are flexible because the fiber substrate is made of polyurethane. Therefore, the fibers demonstrated in this study could make it possible to control the electric properties by modifying the nanofiber morphology.

  • An Electrochemical DNA Sensing System Using Modified Nanoparticle Probes for Detecting Methicillin-Resistant Staphylococcus aureus.
    Sakamoto Hiroaki;Amano Yoshihisa;Satomura Takenori;Suye Shin-Ichiro
    Methods in molecular biology (Clifton, N.J.) 1572 2017
    :We have developed a novel, highly sensitive, biosensing system for detecting methicillin-resistant Staphylococcus aureus (MRSA). The system employs gold nanoparticles (AuNPs), magnetic nanoparticles (mNPs), and an electrochemical detection method. We have designed and synthesized ferrocene- and single-stranded DNA-conjugated nanoparticles that hybridize to MRSA DNA. Hybridized complexes are easily separated by taking advantage of mNPs. A current response could be obtained through the oxidation of ferrocene on the AuNP surface when a constant potential of +250 mV vs. Ag/AgCl is applied. The enzymatic reaction of L-proline dehydrogenase provides high signal amplification. This sensing system, using a nanoparticle-modified probe, has the ability to detect 10 pM of genomic DNA from MRSA without amplification by the polymerase chain reaction. Current responses are linearly related to the amount of genomic DNA in the range of 10-166 pM. Selectivity is confirmed by demonstrating that this sensing system could distinguish MRSA from Staphylococcus aureus (SA) DNA.
  • Development of a high-sensitive electrochemical detector with micro-stirrer driven by surface acoustic waves
    Sakamoto, Hiroaki;Kitanishi, Hiroki;Amaya, Satoshi;Saiki, Tsunemasa;Utsumi, Yuichi;Suye, Shin-ichiro
    SENSORS AND ACTUATORS B-CHEMICAL 260 705-709 May  2018
  • Native collagen hydrogel nanofibres with anisotropic structure using core-shell electrospinning
    Wakuda, Yuka;Nishimoto, Shohei;Suye, Shin-ichiro;Fujita, Satoshi
    SCIENTIFIC REPORTS 8 Apr.  2018
  • d-Lactate electrochemical biosensor prepared by immobilization of thermostable?dye-linked d-lactate dehydrogenase from Candidatus Caldiarchaeum?subterraneum.
    Satomura Takenori;Hayashi Junji;Sakamoto Hiroaki;Nunoura Takuro;Takaki Yoshihiro;Takai Ken;Takami Hideto;Ohshima Toshihisa;Sakuraba Haruhiko;Suye Shin-Ichiro
    Journal of bioscience and bioengineering 2018
    :A stable d-lactate electrochemical sensing system was developed using a dye-linked d-lactate dehydrogenase (Dye-DLDH) from an uncultivated thermophilic archaeon, Candidatus Caldiarchaeum subterraneum. To develop the system, the putative gene encoding the Dye-DLDH from Ca. Caldiarchaeum subterraneum was overexpressed in Escherichia coli, and the expressed product was purified. The recombinant enzyme was a highly thermostable Dye-DLDH that retained full activity after incubation for 10 min at 70°C. The electrode for detection of d-lactate was prepared by immobilizing the thermostable Dye-DLDH and multi-walled carbon nanotube (MWCNT) within Nafion membrane. The electrocatalytic response of the electrode was clearly observed upon exposure to d-lactate. The electrode response to d-lactate was linear within the concentration range of 0.03-2.5 mM, and it showed little reduction in responsiveness after 50 days. This is the first report describing a d-lactate sensing system using a thermostable Dye-DLDH.
  • Characterization of protein adsorption on stretched polyurethane nanofibers prepared by electrospinning
    Morita Yuko, Sakamoto Hiroaki, Suye Shin-ichiro
    RSC Advances 260 705-709 Dec.  2017 Refereed
  • Development of Biofuel Cell Using a Complex of Highly Oriented Immobilized His-Tagged Enzyme and Carbon Nanotube Surface Through a Pyrene Derivative.
    Sakamoto Hiroaki;Koto Ayako;Takamura Ei-Ichiro;Asakawa Hitoshi;Fukuma Takeshi;Satomura Takenori;Suye Shin-Ichiro
    Journal of nanoscience and nanotechnology 19(6) 2019
    :For increasing the output of biofuel cells, increasing the cooperation between enzyme reaction and electron transfer on the electrode surface is essential. Highly oriented immobilization of enzymes onto a carbon nanotube (CNT) with a large specific surface area and excellent conductivity would increase the potential for their application as biosensors and biofuel cells, by utilizing the electron transfer between the electrode-molecular layer. In this study, we prepared a CNT-enzyme complex with highly oriented immobilization of enzyme onto the CNT surface. The complex showed excellent electrical characteristics, and could be used to develop biodevices that enable efficient electron transfer. Multi-walled carbon nanotubes (MWCNT) were dispersed by pyrene butyric acid N-hydroxysuccinimide ester, and then N-(5-amino-1-carboxypentyl) iminodiacetic acid (AB-NTA) and NiCl₂ were added to modify the NTA-Ni2+ complex on the CNT surface. Pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) was immobilized on the CNT surface through a genetically introduced His-tag. Formation of the MWCNT-enzyme complex was confirmed by monitoring the catalytic current electrochemically to indicate the enzymatic activity. PQQ-GDH was also immobilized onto a highly ordered pyrolytic graphite surface using a similar process, and the enzyme monolayer was visualized by atomic force microscopy to confirm its structural properties. A biofuel cell was constructed using the prepared CNT-enzyme complex and output evaluation was carried out. As a result, an output of 32 μW/cm² could be obtained without mediators.
  • Direct cryopreservation of adherent cells on an elastic nanofiber sheet featuring a low glass-transition temperature
    Batnyam, Onon;Suye, Shin-ichiro;Fujita, Satoshi
    RSC ADVANCES 7(81) 51264-51271 2017
  • Characterization of protein adsorption on stretched polyurethane nanofibers prepared by electrospinning
    Morita, Yuko;Sakamoto, Hiroaki;Suye, Shin-ichiro
    RSC ADVANCES 7(89) 56484-56488 2017
  • D-Lactate electrochemical biosensor prepared by immobilization of thermostable dye-linked D-lactate dehydrogenase from Candidatus Caldiarchaeum subterraneum
    Satomura, Takenori;Hayashi, Junji;Sakamoto, Hiroaki;Nunoura, Takuro;Takaki, Yoshihiro;Takai, Ken;Takami, Hideto;Ohshima, Toshihisa;Sakuraba, Haruhiko;Suye, Shin-ichiro
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING 126(4) 425-430 Oct.  2018
  • A L-proline/O-2 biofuel cell using L-proline dehydrogenase (LPDH) from Aeropyrum pernix
    Tonooka, Aina;Komatsu, Tomohiro;Tanaka, Shino;Sakamoto, Hiroaki;Satomura, Takenori;Suye, Shin-ichiro
    MOLECULAR BIOLOGY REPORTS 45(6) 1821-1825 Dec.  2018
  • Development of Biofuel Cell Using a Complex of Highly Oriented Immobilized His-Tagged Enzyme and Carbon Nanotube Surface Through a Pyrene Derivative
    Sakamoto, Hiroaki;Koto, Ayako;Takamura, Ei-Ichiro;Asakawa, Hitoshi;Fukuma, Takeshi;Satomura, Takenori;Suye, Shin-Ichiro
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 19(6) 3551-3557 Jun.  2019
  • Design of a multi-enzyme reaction on an electrode surface for an L-glutamate biofuel anode.
    Sakamoto Hiroaki;Komatsu Tomohiro;Yamasaki Koji;Satomura Takenori;Suye Shin-Ichiro
    Biotechnology letters 39(2) 2017
    OBJECTIVES:To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH.;RESULTS:Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. The ratio of the immobilized enzymes was an important factor affecting the efficiency of the system; thus, we quantified the amounts of immobilized enzyme using a quartz crystal microbalance to further evaluate the electrochemical reaction. The electrochemical reaction proceeded efficiently when approximately equimolar amounts of the enzyme were on the electrode. The largest oxidation peak current increase (171 nA) was observed under these conditions.;CONCLUSION:Efficient multi-enzyme reaction on the electrode surface has been achieved which is applicable for biofuel cell application.
  • Native collagen hydrogel nanofibres with anisotropic structure using core-shell electrospinning.
    Wakuda Yuka;Nishimoto Shohei;Suye Shin-Ichiro;Fujita Satoshi
    Scientific reports 8(1) 2018
    :Collagen hydrogel is a popular extracellular matrix (ECM) material in regenerative medicine and has an isotropic structure. In contrast, native ECM has an anisotropic structure. Electrospinning of collagen dissolved in organic solvents is widely used for fabricating anisotropic collagen nanofibres; however, such fibres are water-soluble and require cross-linking before use as scaffolds for cell culture. Herein, electrospinning using a core-shell nozzle was employed to spin an aqueous acidic solution of collagen and encapsulate it within a shell of polyvinylpyrrolidone (PVP). Subsequently, the core collagen was gelled, and the shell PVP was washed away using a basic ethanol solution to yield anisotropic collagen hydrogel nanofibres. Immunostaining and Fourier transform infrared spectroscopy revealed that the obtained fibres were composed of collagen, and surface PVP was removed completely. Circular dichroism measurements confirmed that the fibres exhibited the triple helical structure characteristic of collagen. Human umbilical vein endothelial cells cultured on the collagen hydrogel fibres were oriented along the fibre direction. Hence, this method is suitable for fabricating fibrous anisotropic collagen hydrogels without chemical and thermal cross-linking, and can facilitate the development of safe medical materials with anisotropy similar to that of native ECM.
  • A L-proline/O2 biofuel cell using L-proline dehydrogenase (LPDH) from Aeropyrum pernix.
    Tonooka Aina;Komatsu Tomohiro;Tanaka Shino;Sakamoto Hiroaki;Satomura Takenori;Suye Shin-Ichiro
    Molecular biology reports 45(6) 2018
    :To utilize amino acids from food waste as an energy source, L-proline/O2 biofuel cell was constructed using a stable enzyme from hyperthermophilic archaeon for long-term operation. On the anode, the electrocatalytic oxidation of L-proline by L-proline dehydrogenase from Aeropyrum pernix was observed in the presence of ferrocenecarboxylic acid as mediator. On the cathode, electrocatalytic oxygen reduction was detected. Ketjenblack modification of carbon cloth substrate increased the current density due to increased laccase loading and enhanced electron transfer reaction. The biofuel cell using these electrodes achieved a current density of 6.00 µA/cm2. We successfully constructed the first biofuel cell that generates power from L-proline.
  • Enzymological characteristics of a novel archaeal dye-linked D-lactate dehydrogenase showing loose binding of FAD.
    Satomura Takenori;Hayashi Junji;Ohshida Tatsuya;Sakuraba Haruhiko;Ohshima Toshihisa;Suye Shin-Ichiro
    Extremophiles : life under extreme conditions 22(6) 2018
    :A gene-encoding a dye-linked D-lactate dehydrogenase (Dye-DLDH) homolog was identified in the genome of the hyperthermophilic archaeon Thermoproteus tenax. The gene was expressed in Escherichia coli and the product was purified to homogeneity. The recombinant protein exhibited highly thermostable Dye-DLDH activity. To date, four types of Dye-DLDH have been identified in hyperthermophilic archaea (in Aeropyrum pernix, Sulfolobus tokodaii, Archaeoglobus fulgidus, and Candidatus Caldiarchaeum subterraneum). The amino acid sequence of T. tenax Dye-DLDH showed the highest similarity (45%) to A. pernix Dye-DLDH, but neither contained a known FAD-binding motif. Nonetheless, both homologs required FAD for enzymatic activity, suggesting that FAD binds loosely to the enzyme and is easily released unlike in other Dye-DLDHs. Our findings indicate that Dye-DLDHs from T. tenax and A. pernix are a novel type of Dye-DLDH characterized by loose binding of FAD.
  • Effects of multicopper oxidase orientation in multiwalled carbon nanotube biocathodes on direct electron transfer.
    Takamura Eiichiro;Nakamura Takuto;Sakamoto Hiroaki;Satomura Takenori;Sakuraba Haruhiko;Ohshima Toshihisa;Suye Shin-Ichiro
    Biotechnology and applied biochemistry 2018
    :In this study, multicopper oxidase (MCO) was immobilized on multiwalled carbon nanotubes (MWCNTs) at two different orientations, and the electrochemical properties of the resulting cathodes were investigated. Using N- or C-terminal His-tagged MCO and MWCNTs, we constructed two types of cathodes. We assumed that the distance between the type 1 (T1)Cu of the C-terminal His-tagged MCO and the MWCNT surface was lesser than that between the T1Cu of the N-terminal His-tagged MCO and the MWCNT surface. In addition, in the C-terminal His-tagged MCO, T1Cu was expected to be closer to the MWCNT surface than the type 2/type 3 Cu site. The current density of the modified electrode with a C-terminal His-tagged MCO immobilized on an MWCNT surface was 1.3-fold higher than that of the electrode with an N-terminal His-tagged MCO immobilized on an MWCNT surface. In addition, the amount of H2 O2 produced by the N-terminal His-tagged MCO immobilized MWCNT modified electrodes was 2.3-fold higher than that produced by the C-terminal His-tagged MCO immobilized MWCNT electrodes. In direct electron transfer (DET)-type biocathodes, both the MCO orientation and the distance between the T1Cu of MCO and the electrode surface are important. The authors succeeded in constructing highly efficient DET-type electrodes.

Books etc

  • Molecular film assembled detection system for biosensors by layer-by-layer adsorption
    S.Suye, H.Zheng
    Molecular film assembled detection system for biosensors by layer-by-layer adsorption
     Apr.  2007

Conference Activities & Talks

  • 1B02 カーボンナノチューブを含む交互積層ナノデバイスを用いた有害微生物の高感度検出
    末 信一朗, 桑田典明
     Jun.  2012