FacultyBiomedical Imaging Research Center
Teacher OrganizationBiomedical Imaging Research Center
Education and
 Research Organization
Biomedical Imaging Research Center
PositionAssociate Professor
Last Updated: 19/12/05 15:50

Researcher Profile & Settings


    MAKINO Akira


  •  Biomedical Imaging Research Center Associate Professor


  • Apr. 2003Mar. 2006Kyoto University 材料化学専攻
  • Apr. 2001Mar. 2003Kyoto University 材料化学専攻
  • Apr. 1997Mar. 2001Kyoto University Industrial Chemistry

Association Memberships

    The Pharmaceutical Society of Japan member,

Research Activities

Published Papers

  • Inflammation-induced synergetic enhancement of nanoparticle treatments with DOXILR and 90Y-Lactosome for orthotopic mammary tumor
    Kurihara K, Ueda M, Hara I, Hara E, Sano K, Makino A, Ozeki E, Yamamoto F, Saji H, Togashi K, Kimura S
    J Nanopart Res 18 137 May  2016 Refereed
  • Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors
    Kanazaki K, Sano K, Makino A, Homma T, Ono M, Saji H
    Sci Rep 6 33798 Sep.  2016 Refereed
  • 18F-Labeled Pyrido[3,4-d]pyrimidine as an Effective Probe for Imaging of L858R Mutant Epidermal Growth Factor Receptor
    Kimura H, Okuda H, Ishiguro M, Arimitsu K, Makino A, Nishii R, Miyazaki A, Yagi Y, Watanabe H, Kawasaki I, Ono M, Saji H
    ACS Med Chem Lett 8(4) 418-422 Mar.  2017 Refereed
  • Faeasibility of poly(ethylene glycol) derivatives as diagnostic drug carrier for tumor imaging
    K.Kanazaki, K.Sano, A.Makino, F.Yamauchi, A.Takahashi, T.Homma, M.Ono, H.Saji
    J Control Release 226 115-123 Mar.  2016 Refereed
  • Development of novel PET probes targeting phosphatidylinositol 3-kinase (PI3K) in tumors
    A.Makino, T.Arai, M.Hirata, M.Ono, Y.Ohmomo, H.Saji
    Nucl Med Biol 43(1) 101-107 Jan.  2016 Refereed
  • Development of anti-HER2 fragment antibody conjugated to iron oxide nanoparticles for in vivo HER2-targeted photoacoustic tumor imaging
    K.Kanazaki, K.Sano, A.Makino, Y.Shimizu, F.Yamauchi, S.Ogawa, N.Ding, T.Yano, T.Temma, M.Ono, H.Saji
    Nanomedicine: NBM 11(8) 2051-2060 Nov.  2015 Refereed
  • Development of human serum albumin conjugated with near-infrared dye for photoacoustic tumor imaging
    Kengo Kanazaki, Kohei Sano, Akira Makino, Atsushi Takahashi, Jun Deguchi, Manami Ohashi, Takashi Temma, Masahiro Ono, Hideo Saji
    Journal of biomedical optics 19(9) 96002 Sep.  2014 Refereed
  • Factors Influencing in Vivo Disposition of Polymeric Micelles on Multiple Administrations
    Eri Hara, Motoki Ueda, Akira Makino, Isao Hara, Eiichi Ozeki, Shunsaku Kimura
    ACS Med. Chem. Lett. 5(8) 873-877 Aug.  2014 Refereed
  • Morphology control of molecular assemblies prepared from bio-based amphiphilic polymers with a helical hydrophobic unit and application as nanocarriers for contrast agents and/or drug delivery
    Akira Makino
    Polymer Journal 46(11) 783-791 Aug.  2014 Refereed
  • In vivo imaging of membrane type-1 matrix metalloproteinase with a novel activatable near-infrared fluorescence probe
    Yoichi Shimizu, Takashi Temma, Isao Hara, Akira Makino, Naoya Kondo, Eiichi Ozeki, Masahiro Ono, Hideo Saji
    Cancer Science 105(8) 1056-1062 Aug.  2014 Refereed
  • Suppressive immune response of poly-(sarcosine) chains in peptide-nanosheets in contrast to polymeric micelles
    Eri Hara, Motoki Ueda, Akira Makino, Cheol Joo Kim, Isao Hara, Eiichi Ozeki, Shunsaku Kimura
    Journal of Peptide Science 20(7) 570-577 Jul.  2014 Refereed
  • Morphology control between twisted ribbon, helical ribbon, and nanotube self-assemblies with His-containing helical peptides in response to pH change
    Akihiro Uesaka, Motoki Ueda, Akira Makino, Tomoya Imai, Junji Sugiyama, Shunsaku Kimura
    Langmuir 30(4) 1022-1028 Feb.  2014 Refereed
  • Size Control of Core-Shell-type Polymeric Micelle with a Nanometer Precision
    Akira Makino, Eri Hara, Isao Hara, Eiichi Ozeki, Shunsaku Kimura
    Langmuir 30(2) 669-674 Jan.  2014 Refereed
  • Solid Tumor-Targeting Theranostic Polymer Nanoparticle in Nuclear Medicinal Fields
    Akira Makino, Shunsaku Kimura
    The Scientific World Journal 2014 Article ID 424513 Jan.  2014 Refereed
  • Micelle-based activatable probe for in vivo near-infrared optical imaging of cancer biomolecules
    Yoichi Shimizu, Takashi Temma, Isao Hara, Akira Makino, Ryo Yamahara, Eiichi Ozeki, Masahiro Ono, Hideo Saji
    Nanomedicine: Nanotechnology, Biology and Medicine 10(1) 187-195 Jan.  2014 Refereed
  • The Potential of o-Bromo-trans-decalinvesamicol as a New PET Ligand for Vesicular Acetylcholine Transporter Imaging
    Mohammad Anwar-ul Azim, Takashi Kozaka, Izumi Uno, Daisuke Miwa, Yoji Kitamura, Kazuma Ogawa, Akira Makino, Yasushi Kiyono, Kazuhiro Shiba
    Synapse 68(10) 445-453 Jan.  2014 Refereed
  • Radionuclide therapy using nanoparticle of 131I-Lactosome in combination with percutaneous ethanol injection therapy
    Eri Hara, Akira Makino, Kensuke Kurihara, Motoki Ueda, Isao Hara, Takashi Kawabe, Fumihiko Yamamoto, Eiichi Ozeki, Kaori Togashi, Shunsaku Kimura
    Journal of Nanoparticle Research 15(12) Article:2131 Nov.  2013 Refereed
  • アップコンバージョンナノ粒子を使った光線力学療法の新展開
    Farumashia 49(9) 899 Sep.  2013 Refereed
  • Evasion from accelerated blood clearance of nanocarrier named as “Lactosome” induced by excessive administration of Lactosome
    Eri Hara, Akira Makino, Kensuke Kurihara, Manabu Sugai, Akira Shimizu, Isao Hara, Eiichi Ozeki, Shunsaku Kimura
    Biochimica et Biophysica Acta (BBA) - General Subjects 1830(8) 4046-4052 Aug.  2013 Refereed
  • Versatile peptide rafts for conjugate morphologies by self-assembling amphiphilic helical peptides
    Motoki Ueda, Akira Makino, Tomoya Imai, Junji Sugiyama, Shunsaku Kimura
    Polymer Journal 45(5) 509-515 May  2013 Refereed
  • Radiosynthesis and initial evaluation of 18F labeled nanocarrier composed of poly(L-lactic acid)-block-poly(sarcosine) amphiphilic polydepsipeptide
    Fumihiko Yamamoto, Ryo Yamahara, Akira Makino, Kensuke Kurihara, Hideo Tsukada, Eri Hara, Isao Hara, Shinae Kizaki-Kondoh, Y. Ohkubo, Eiichi Ozeki, Shunsaku Kimura
    Nuclear Medicine and Biology 40(3) 387-394 Apr.  2013 Refereed
  • Assessment of the tumor redox status in head and neck cancer by 62Cu-ATSM PET.
    Tsujikawa T, Asahi S, Oh M, Sato Y, Narita N, Makino A, Kiyono Y, Tsuchida T, Kimura H, Fujieda S, Okazawa H.
    PLoS ONE 11(5) e0155635 May  2016 Refereed
    PURPOSE:Tumor redox is an important factor for cancer progression, resistance to treatments, and a poor prognosis. The aim of the present study was to define tumor redox (over-reduction) using 62Cu-diacetyl-bis(N4-methylthiosemicarbazone) (62Cu-ATSM) PET and compare its prognostic potential in head and neck cancer (HNC) with that of 2-deoxy-2-[18F]fluoro-D-glucose (18F-FDG).;METHODS:Thirty HNC patients (stage II-IV) underwent pretreatment 62Cu-ATSM and 18F-FDG PET scans. Maximum standardized uptake values (SUVATSM and SUVFDG) and tumor-to-muscle activity concentration ratios (TMRATSM and TMRFDG) were measured. Reductive-tumor-volume (RTV) was then determined at four thresholds (40%, 50%, 60%, and 70% SUVATSM), and total-lesion-reduction (TLR) was calculated as the product of the mean SUV and RTV for 62Cu-ATSM. In 18F-FDG, metabolic-tumor-volume (MTV) and total-lesion-glycolysis (TLG) were obtained at a threshold of 40%. A ROC analysis was performed to determine % thresholds for RTV and TLR showing the best predictive performance, and these were then used to determine the optimal cut-off values to stratify patients for each parameter. Progression-free-survival (PFS) and cause-specific-survival (CSS) were evaluated by the Kaplan-Meier method.;RESULTS:The means ± standard deviations of PFS and CSS periods were 16.4±13.4 and 19.2±12.4 months, respectively. A ROC analysis determined that the 70% SUVATSM threshold for RTV and TLR was the best for predicting disease progression and cancer death. Optimal cut-offs for each index were SUVATSM = 3.6, SUVFDG = 7.9, TMRATSM = 3.2, TMRFDG = 5.6, RTV = 2.9, MTV = 8.1, TLR = 14.0, and TLG = 36.5. When the cut-offs for TMRATSM and TLR were set as described above in 62Cu-ATSM PET, patients with higher TMRATSM (p = 0.03) and greater TLR (p = 0.02) showed significantly worse PFS, while patients with greater TLR had significantly worse CSS (p = 0.02). Only MTV in 18F-FDG PET predicted differences in PSF and CSS (p = 0.03 and p = 0.03, respectively).;CONCLUSION:Tumor redox parameters measured by 62Cu-ATSM PET may be determinants of HNC patient outcomes and help define optimal patient-specific treatments.
  • Indocyanine Green-Labeled Polysarcosine for in Vivo Photoacoustic Tumor Imaging
    Sano K, Ohashi M, Kanazaki K, Makino A, Ding N, Deguchi J, Kanada Y, Ono M, Saji H
    Bioconjug Chem 28(4) 1024-1030 Apr.  2017 Refereed
    :Photoacoustic (PA) imaging has been considered an attractive imaging modality for sensitive and in-depth imaging of biomolecules with a high resolution in vivo. PA imaging probes utilizing fluorescence dyes, including indocyanine green (ICG), have been proposed to enhance PA signal intensity. On the other hand, nanomicelles modified with polysarcosine (PSar), a biocompatible hydrophilic polymer, on their surface have previously achieved rapid tumor uptake, suggesting active transport of PSar into tumor tissues. Thus, we hypothesized that PSar-based materials might be utilized as diagnostic probes for targeting tumors and therefore evaluated the potential of PSar labeled with an ICG derivative, ICG-PSar, as a PA imaging probe for targeting cancer. In this study, ICG-PSars with differing molecular weights (10, 20, and 30 kDa) were synthesized. In vitro cellular uptake studies using ICG-PSar demonstrated rapid uptake in colon26 tumor cells partially via macropinocytosis-mediated endocytosis. In vivo fluorescence imaging and biodistribution study indicated that ICG-PSar30k exhibited high accumulation in the tumor (8.4% dose/g), with high tumor-to-blood ratios reaching 4.6 at 24 h post injection of the probe.
  • PET probe detecting non-small cell lung cancer susceptible to epidermal growth factor receptor tyrosine kinase inhibitor therapy
    Makino A, Miyazaki A, Tomoike A, Kimura H, Arimitsu K, Hirata M, Ohmomo Y, Nishii R, Okazawa H, Kiyono Y, Ono M, Saji H
    Bioorg Med Chem 26(8) 1609-1613 May  2018 Refereed
  • Evaluation of Ga-DOTA-(D-Asp)n as bone imaging agents: D-aspartic acid peptides as carriers to bone
    Ogawa K, Ishizaki A, Takai K, Kitamura Y, Makino A, Kozuka T, Kiyono Y, Shiba K, Odani A
    Sci Rep 7(1) 13971 Oct.  2017 Refereed
  • Radiobrominated benzimidazole-quinoline derivatives as Platelet-derived growth factor receptor beta (PDGFRβ) imaging probes
    Effendi N, Mishiro K, Takarada T, Makino A, Yamada D, Kitamura Y, Shiba K, Kiyono Y, Odani A, Ogawa K
    Scientific Reports 8(1) 10369 Jul.  2018 Refereed
  • Development of a novel radiobromine-labeled sigma-1 receptor imaging probe
    Ogawa K, Masuda R, Mizuno Y, Makino A, Kozaka T, Kitamura Y, Kiyono Y, Shiba K, Odani A
    Nuclear Medicine and Biology 61 28-35 Jun.  2018 Refereed
  • 18F-FLT PET/MRI for bone marrow failure syndrome-initial experience
    Tsujikawa T, Tasaki T, Hosono N, Mori T, Makino A, Kiyono Y, Zanotti-Fregonara P, Yamauchi T, Okazawa H
    Eur J Nucl Med Mol Img Re 9(1) 26 Feb.  2019 Refereed
  • No significant difference found in PET/MRI CBF values reconstructed with CT-atlas-based and ZTE MR attenuation correction
    Okazawa H, Tsujikawa T, Higashino Y, Kikuta K, Mori T, Makino A, Kiyono Y
    Eur J Nucl Med Mol Img Re 9(1) 26 Mar.  2019 Refereed
  • Comparison of Radioiodine- or Radiobromine-Labeled RGD Peptides between Direct and Indirect Labeling Methods
    Ogawa K, Takeda T, Yokokawa M, Yu J, Makino A, Kiyono Y, Shiba K, Kinuya S, Odani A
    Chemical and Pharmaceutical Bulletin 66(6) 651-659 Jun.  2018 Refereed

Conference Activities & Talks

  • Development of a PET probe detecting tumors, which are responsive to gefitinib treatment
    Makino A, Miyazaki A, Kimura H, Hirata M, Ohmomo Y, Nishii R, Okazawa H, Kiyono Y, Ono M, Saji H
    Society of Nuclear Medicine and Molecular Imaging 2016 Annual Meeting Jun.  2016 Society of Nuclear Medicine
  • Polymeric micelle type dual imaging probe for PET and optical imaging techniques
    A.Makino, A.Tomoike, H.Kimura, M.Ono, G.Nishimura, Y.Kiyono, H.Saji
     Sep.  2014
  • In vivo HER2-Targeted Photoacoustic Tumor Imaging Using Anti-HER2 Fragment Antibody Conjugated to Iron Oxide Nanoparticle
    K.Sano, K.Kanazaki, A.Makino, Y.Shimizu, F.Yamauchi, S.Ogawa, T.Yano, T.Temma, M.Ono, H.Saji
     Sep.  2014
  • Synthesis and initial evaluation of radioiodine labeled A3B-type Lactosome as an imaging probe of tumor and/or inflammation
    F.Yamamoto, M.Kimura, A.Makino, Y.Yamamoto, Y.Saito, E.Ozeki, S.Kimura, Y.Ohkubo
    Society of Nuclear Medicine and Molecular Imaging Annual Meeting 2014 Jun.  2014
  • Volume-based hypoxic parameters of 62Cu-ATSM PET as prognostic indicators in head and neck cancer
    T.Tsujikawa, S.Asahi, M. Oh, Y. Sato, T.Tsuchida, A. Makino, T.Mori, Y.Kiyono, S.Fujieda, H.Okazawa
    The 61st Annual Meeting of The Society of Nuclear Medicine Jun.  2014
  • Evaluation of the polymeric micelle dynamics in vivo by using different two radioisotope modification approaches
    A.Makino, A.Tomoike, M.Ono, S.Kimura, H.Saji
     Mar.  2014
  • Evaluation of radiolabeled o-bromo-decalinvesamicol (OBDV) as a PET ligand for the Vesicular Acetylcholine Transporter (VAChT).
    Mohammad Anwar-ul Azim, T.Kozaka, I.Uno, D.Miwa, Y.Kitamura, K.Ogawa, K.Kawai, A.Makino, Y.Kiyono, K.Shiba
     Mar.  2014
  • HPLC and HPLC-ESI-MS/MS methods for the determination of 2-acetamido-2-deoxy-D-glucopyranose analogues
    M.E.Martinez, Y.Tokunaga, T.Mori, A.Makino, S.Noriki, K.Inai, H.Okazawa, Y.Kiyono
     Jan.  2014
  • Development of a novel PET tracer targeting phospatidylinositol 3-kinase (PI3K) in tumor
    A.Makino, T.Arai, T.Temma, M.Hirata, M.Ono, Y.Ohmomo, H.Saji
     Jan.  2014
  • Biodistribution of self-assembled nanoparticulate, Lactosome" by different two radioisotope modification methods"
    A.Makino, A.Tomoike, H.Saji, Y.Kiyono
     Sep.  2013
  • Assessment of hormone sensitivity in uterine leiomyoma patients using 18F-FES PET
    T.Tsujikawa, Y.Chino, A.Shinagawa, M.Yamamoto, A.Makino, T.Mori, T.Kurokawa, Y.Kiyono, Y.Yoshida, H.Okazawa
    SNMMI 2015 Annual Meeting
  • Evaluation of a new precursor for radiosynthesis of 2-deoxy-2-[18F]fluoroacetamido-D-glucopyranose
    ME. Martinez, Y.Tokunaga, T.Mori, A.Makino, H.Okazawa, Y.Kiyono
    10th Annual Meeting of Japanese Society for Molecular Imaging May  2015
  • Evaluation of nigrostriatal oxidative stress intensity in patients with Parkinson’s disease using [Cu-62]ATSM PET and FP-CIT SPECT.
    H.Okazawa, M.Ikawa, T.Tsujikawa , T.Mori, A.Makino, Y.Kiyono, M.Yoneda
    Annual Congress of the European Association of Nuclear Medicine (EANM2015)  Oct.  2015
  • Development of a low cost multipurpose system for F-18 labeled PET probe synthesis
    T.Mori, M.Kunieda, H.Okazawa, Y.Kiyono
    The 62nd Annual Meeting of the Society of Nuclear Medicine Jun.  2015
  • PET probe detecting acquired drug resistance against EGFR targeted molecular therapy
    A.Makino, A.Miyazaki, A.Tomoike, H.Kimura, M.Hirata, Y.Ohmomo, M.Ono, H.Okazawa, Y.Kiyono, H.Saji
    Annual Congress of the European Association of Nuclear Medicine 2015 Oct.  2015
  • Evaluation of in vivo dynamics of the polymeric micelle by using two different radioisotope modification approaches
    A.Makino, A.Tomoike, M.Ono, Y.Kiyono, H.Okazawa, H.Saji
    The 9th Japan-China Joint Seminar on Radiopharmaceutical Chemistry Nov.  2015
  • Micelle type dual imaging probe for positron and near-infrared fluorescence imagings
    A.Makino, A.Tomoike, H.Kimura, M.Ono, H.Okazawa, Y.Kiyono, H.Saji
    The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015) Dec.  2015
  • Predictive value of 3'-deoxy-3'-18F-fluorothymidine uptake in colorectal cancer treated with proton irradiation under hypoxic condition.
    Kiyono Y, Murata M, Makino A, Kume K, Mori T, Asai T, Okazawa H
    The 64th Annual Meeting of The Society of Nuclear Medicine Jun.  2017
  • Development of a new fluorine-18 labelled probe targeting norepinephrine transporter
    Makino A, Martinez ME, Okazawa H, Kiyono Y
    22nd International Symposium on Radiopharmaceutical Sciences(ISRS2017) May  2017
  • Prognostic value of FES PET in patients with endometrial cancer
    Tsujikawa T, Yamada S, Tsuyoshi H, Kurokawa T, Shinagawa A, Chino Y, Mori T, Makino A, Kiyono Y, Yoshida Y
     May  2017
  • Assessment of bone marrow proliferation in bone marrow failure syndrome by FLT-PET/MRI
     Nov.  2018
  • Development of nanoparticles for internal radiation therapy
     Nov.  2018
  • Examination on simple preparation method of Cu-64 labeled nanoparticle
     May  2018
  • Development of thermo-sensitive polymer micelle for direct injection at the targeted tumor region
    Makino A, manabe T, Asai T, Okazawa H, Kiyono Y
    256th ACS National Meeting Aug.  2018 American Chemical Society
  • Predictive value of 18F-FDG and 18F-FLT uptake in colorectal cancer treated with charged particle irradiation under hypoxic condition
    Kiyono Y, Makino A,Kume K,Mori T,Asai T, Okazawa H
    The 65th Annual Meeting of The Society of Nuclear Medicine Jun.  2018
  • Changes in the accumulation of 18F-FDG and 18F-FLT in colorectal cancer cells treated with charged particle irradiation under hypoxic condition
    Kiyono Y, Kume K, Makino A, Mori T, Asai T, Okazawa H
    The 12th World Congress of the World Federation of Nuclear Medicine and Biology (WFNMB 2018) Apr.  2018