FUKAZAWA Yugo

FacultyMorphological and Physiological Sciences, Brain Structure and Function
Teacher OrganizationMorphological and Physiological Sciences
Education and
 Research Organization
Faculty of Medical Sciences, School of Medicine
PositionProfessor
Last Updated: 20/10/22 18:25

Researcher Profile & Settings

Name

    FUKAZAWA Yugo

Affiliation

  •  Morphological and Physiological Sciences, Brain Structure and Function Professor

Overseas travel history

  • England Oxford Univeristy MRC Jun.  2006- Sep. 2006
  • アメリカ合衆国 カリフォルニア大学バークレー校・統合生物学教室 Nov.  1992- Apr. 1993

Research Activities

Published Papers

  • Microglial SIRPα regulates the emergence of CD11c+ microglia and demyelination damage in white matter
    Sato-Hashimoto Miho;Nozu Tomomi;Toriba Riho;Horikoshi Ayano;Akaike Miho;Kawamoto Kyoko;Hirose Ayaka;Hayashi Yuriko;Nagai Hiromi;Shimizu Wakana;Saiki Ayaka;Ishikawa Tatsuya;Elhanbly Ruwaida;Kotani Takenori;Murata Yoji;Saito Yasuyuki;Naruse Masae;Shibasaki Koji;Oldenborg Per-Arne;Jung Steffen;Matozaki Takashi;Fukazawa Yugo;Ohnishi Hiroshi
    eLife 8 Mar.  2019 Refereed
    :A characteristic subset of microglia expressing CD11c appears in response to brain damage. However, the functional role of CD11c+ microglia, as well as the mechanism of its induction, are poorly understood. Here we report that the genetic ablation of signal regulatory protein α (SIRPα), a membrane protein, induced the emergence of CD11c+ microglia in the brain white matter. Mice lacking CD47, a physiological ligand of SIRPα, and microglia-specific SIRPα-knockout mice exhibited the same phenotype, suggesting that an interaction between microglial SIRPα and CD47 on neighbouring cells suppressed the emergence of CD11c+ microglia. A lack of SIRPα did not cause detectable damage to the white matter, but resulted in the increased expression of genes whose expression is characteristic of the repair phase after demyelination. In addition, cuprizone-induced demyelination was alleviated by the microglia-specific ablation of SIRPα. Thus, microglial SIRPα suppresses the induction of CD11c+ microglia that have the potential to accelerate the repair of damaged white matter.
  • PIP3-Phldb2 is crucial for LTP regulating synaptic NMDA and AMPA receptor density and PSD95 turnover
    Xie Min-Jue;Ishikawa Yasuyuki;Yagi Hideshi;Iguchi Tokuichi;Oka Yuichiro;Kuroda Kazuki;Iwata Keiko;Kiyonari Hiroshi;Matsuda Shinji;Matsuzaki Hideo;Yuzaki Michisuke;Fukazawa Yugo;Sato Makoto
    Scientific reports 9(1) Mar.  2019 Refereed
    :The essential involvement of phosphoinositides in synaptic plasticity is well-established, but incomplete knowledge of the downstream molecular entities prevents us from understanding their signalling cascades completely. Here, we determined that Phldb2, of which pleckstrin-homology domain is highly sensitive to PIP3, functions as a phosphoinositide-signalling mediator for synaptic plasticity. BDNF application caused Phldb2 recruitment toward postsynaptic membrane in dendritic spines, whereas PI3K inhibition resulted in its reduced accumulation. Phldb2 bound to postsynaptic scaffolding molecule PSD-95 and was crucial for localization and turnover of PSD-95 in the spine. Phldb2 also bound to GluA1 and GluA2. Phldb2 was indispensable for the interaction between NMDA receptors and CaMKII, and the synaptic density of AMPA receptors. Therefore, PIP3-responsive Phldb2 is pivotal for induction and maintenance of LTP. Memory formation was impaired in our Phldb2-/- mice.
  • SK2 Channels Associate With mGlu1α Receptors and CaV2.1 Channels in Purkinje Cells
    Lujan, Rafael;Aguado, Carolina;Ciruela, Francisco;Morato Arus, Xavier;Martin-Belmonte, Alejandro;Alfaro-Ruiz, Rocio;Martinez-Gomez, Jesus;de la Ossa, Luis;Watanabe, Masahiko;Adelman, John P.;Shigemoto, Ryuichi;Fukazawa, Yugo
    FRONTIERS IN CELLULAR NEUROSCIENCE 12 Sep.  2018 Refereed
  • Optogenetic Control of Synaptic AMPA Receptor Endocytosis Reveals Roles of LTD in Motor Learning
    Kakegawa, Wataru;Katoh, Akira;Narumi, Sakae;Miura, Eriko;Motohashi, Junko;Takahashi, Akiyo;Kohda, Kazuhisa;Fukazawa, Yugo;Yuzaki, Michisuke;Matsuda, Shinji
    NEURON 99(5) 985 Sep.  2018 Refereed
  • Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
    Lanjakornsiripan, Darin;Pior, Baek-Jun;Kawaguchi, Daichi;Furutachi, Shohei;Tahara, Tomoaki;Katsuyama, Yu;Suzuki, Yutaka;Fukazawa, Yugo;Gotoh, Yukiko
    NATURE COMMUNICATIONS 9 Apr.  2018 Refereed
  • Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter
    Shinoda, Tomoyasu;Nagasaka, Arata;Inoue, Yasuhiro;Higuchi, Ryo;Minami, Yoshiaki;Kato, Kagayaki;Suzuki, Makoto;Kondo, Takefumi;Kawaue, Takumi;Saito, Kanako;Ueno, Naoto;Fukazawa, Yugo;Nagayama, Masaharu;Miura, Takashi;Adachi, Taiji;Miyata, Takaki
    PLOS BIOLOGY 16(4) Apr.  2018 Refereed
  • Optogenetic activation of non-nociceptive Aβ fibers induces neuropathic pain-like sensory and emotional behaviors after nerve injury in rats
    Ryoichi Tashima, Keisuke Koga, Misuzu Sekine, Kensho Kanehisa, Yuta Kohro, Keiko Tominaga, Katsuyuki Matsushita, Hidetoshi Tozaki-Saitoh, Yugo Fukazawa, Kazuhide Inoue, Hiromu Yawo, Hidemasa Furue, and Makoto Tsuda
    eNeuro 5(1) 2373-2822 Feb.  2018 Refereed
  • Differential association of GABAB receptors with their effector ion channels in Purkinje cells
    Luján R, Aguado C, Ciruela F, Cózar J, Kleindienst D, de la Ossa L, Wickman K, Watanabe M, Shigemoto R, Fukazawa Y
    Brain Structure and Function 223(3) 1565-1587 Nov.  2017 Refereed
  • The number and distribution of AMPA receptor channels containing fast kinetic GluA3 and GluA4 subunits at auditory nerve synapses depend on the target cells
    María E. Rubio, Ko Matsui, Yugo Fukazawa, Naomi Kamasawa, Harumi Harada, Makoto Itakura, Elek Molnár, Manabu Abe, Kenji Sakimura, Ryuichi Shigemoto
    Brain Structure and Function 222(8) 3375-3393 Apr.  2017 Refereed
  • CAPS1 stabilizes the state of readily releasable synaptic vesicles to fusion competence at CA3-CA1 synapses in adult hippocampus.
    Shinoda Yo;Ishii Chiaki;Fukazawa Yugo;Sadakata Tetsushi;Ishii Yuki;Sano Yoshitake;Iwasato Takuji;Itohara Shigeyoshi;Furuichi Teiichi
    Scientific reports 6 Aug.  2016 Refereed
    :Calcium-dependent activator protein for secretion 1 (CAPS1) regulates exocytosis of dense-core vesicles in neuroendocrine cells and of synaptic vesicles in neurons. However, the synaptic function of CAPS1 in the mature brain is unclear because Caps1 knockout (KO) results in neonatal death. Here, using forebrain-specific Caps1 conditional KO (cKO) mice, we demonstrate, for the first time, a critical role of CAPS1 in adult synapses. The amplitude of synaptic transmission at CA3-CA1 synapses was strongly reduced, and paired-pulse facilitation was significantly increased, in acute hippocampal slices from cKO mice compared with control mice, suggesting a perturbation in presynaptic function. Morphological analysis revealed an accumulation of synaptic vesicles in the presynapse without any overall morphological change. Interestingly, however, the percentage of docked vesicles was markedly decreased in the Caps1 cKO. Taken together, our findings suggest that CAPS1 stabilizes the state of readily releasable synaptic vesicles, thereby enhancing neurotransmitter release at hippocampal synapses.
  • Cortical astrocytes rewire somatosensory cortical circuits for peripheral neuropathic pain
    Kim SK, Hayashi H, Ishikawa T, Shibata K, Shigetomi E, Shinozaki Y, Inada H, Roh S-E, Kim SJ, Lee G, Bae H, Woorhouse A, Mikoshiba K, Fukazawa Y, Koizumi S, Nabekura J
    J. Clin. Invest 126(5) 1983-1997 May  2016 Refereed
  • Synapse-dependent and independent mechanisms of thalamocortical axon branching are regulated by neuronal activity
    Matsumoto N, Hoshiko M, Sugo N, Fukazawa Y, Yamamoto N
    Dev. Neurobiol. 76(3) 326-336 Mar.  2016 Refereed
  • Differential expression patterns of K+/Cl- cotransporter 2 in neurons within the superficial spinal dorsal horn of rats
    Javdani, Fariba;Hollo, Krisztina;Hegedus, Krisztina;Kis, Greta;Hegyi, Zoltan;Docs, Klaudia;Kasugai, Yu;Fukazawa, Yugo;Shigemoto, Ryuichi;Antal, Miklos
    J. Comp. Neurol. 523(13) 1967-1983 Sep.  2015 Refereed
  • Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly
    Ohnishi, Takayuki;Yanazawa, Masako;Sasahara, Tomoya;Kitamura, Yasuki;Hiroaki, Hidekazu;Fukazawa, Yugo;Kii, Isao;Nishiyama, Takashi;Kakita, Akiyoshi;Takeda, Hiroyuki;Takeuchi, Akihide;Arai, Yoshie;Ito, Akane;Komura, Hitomi;Hirao, Hajime;Satomura, Kaori;Inoue, Masafumi;Muramatsu, Shin-ichi;Matsui, Ko;Tada, Mari;Sato, Michio;Saijo, Eri;Shigemitsu, Yoshiki;Sakai, Satoko;Umetsu, Yoshitaka;Goda, Natsuko;Takino, Naomi;Takahashi, Hitoshi;Hagiwara, Masatoshi;Sawasaki, Tatsuya;Iwasaki, Genji;Nakamura, Yu;Nabeshima, Yo-ichi;Teplow, David B.;Hoshi, Minako
    Proc. Natl. Acad. Sci. USA. 112(32) E4465-E4474 Aug.  2015 Refereed
  • Different subsynaptic localization of type 1 metabotropic glutamate (mGlu1) receptors at glutamatergic and GABAergic synapses in the rodent cerebellar cortex
    Mansouri M, Kasugai Y, Y.Fukazawa, Bertaso F, Raynaud F, Perroy J, Fagni L, Kaufmann WA, Watanabe M, Shigemoto R, Ferraguti F
    Eur J Neurosci 41 157-167 Nov.  2014 Refereed
  • Coassembly and Coupling of SK2 Channels and mGlu5 Receptors
    Garcia-Negredo G, Soto D, Llorente J, Morato X, Galenkamp KMO, Gomez-Soler M, Fernandez-Dunas V, Watanabe M, Adelman JP, Shigemoto R, Y.Fukazawa, Lujan R, Ciruela F
    J Neurosci 33 14793-14802 Oct.  2014 Refereed
  • Target- and input-dependent organization of AMPA and NMDA receptors in synaptic connections of the cochlear nucleus
    Rubio ME, Y.Fukazawa, Kamasawa N, Clarkson C, Molnar E, Shigemoto R
    J Comp Neurol. 522 4023-4042 Jul.  2014 Refereed
  • Optogenic stimulation of the auditory pathway
    Hernandez VH, Reuter K, Jing Z, Schulz AM, Hoch G, Bartels M, Yawo H, Y.Fukazawa, Augustine GJ, Bamberg E, Kugler S, Salditt T, Strenzke N, Moser T
    J Clin Invest 124 1114-1129 Feb.  2014 Refereed
  • Differential subcellular localization of SK3-containing channels in the hippocampus
    Ballesteros-Merino C, Watanabe M, Shigemoto R, Y.Fukazawa, Adelman JP, Lujan R
    Eur J Neurosci 39 883-892 Jan.  2014 Refereed
  • Optogenic countering of glial acidosis suppresses glial glutamate release and ischemic brain damage
    Beppu K, Sasaki T, Tanaka K, Yamanaka A, Y.Fukazawa, Shigemoto R, Matsui K
    Neuron 81 314-320 Jan.  2014 Refereed
  • Distinct kinetics of synaptic structural plasticity, memory formation and memory decay in massed and spaced learning
    Aziz W, Wang W, Kesaf S, Mohamed AA, Y.Fukazawa, Shigemoto R
    PNAS 111 E194-E202 Jan.  2014 Refereed
  • Distinct cerebellar engrams in short-term and long-term motor learning
    Wang W, Nakadate K, Masugi-Tokita M, Shutoh F, Aziz W, Tarusawa E, Lorincz A, Molnar E, Kesaf S, Li Y-Q, Nagao S, Y.Fukazawa, Shigemoto R
    PNAS 111 E188-E193 Jan.  2014 Refereed
  • Frequency-dependent entrainment of neocortical slow oscillation to repeated optogenetic stimulation in the anesthetized rat
    Kuki T, Ohshiro T, Ito S, Ji Z-G, Y.Fukazawa, Matsuzaka Y, Yawo H, Mushiake H
    Neurosci Res 75 35-45 Nov.  2013 Refereed
  • Paired stimulation between CA3 and CA1 alters excitability of CA3 in the rat hippocampus
    Ohta H, Sakai S, Ito S, Ishizuka T, Y.Fukazawa, Kemuriyama T, Tandai-Hiruma M, Mushiake H, Sato Y, Yawo H, Nishida Y
    Neurosci Letters 534 182-187 Feb.  2013 Refereed
  • Neuroligin-1 controls synaptic abundance of NMDA-type glutamate receptors through extracellular coupling
    Budreck EC, Kwon O-B, Jung JH, Baudouin S, Thommen A, Kim H-S, Y.Fukazawa, Harada H, Tabuchi K, Shigemoto R, Scheiffele P, Kim J-H
    PNAS 110 725-730 Jan.  2013 Refereed
  • Evaluation of glutamate concentration transient in the synaptic cleft
    Budisantoso T, Harada H, Kamasawa N, Y.Fukazawa, Shigemoto R, Matsui K
    J Physiol 591 219-239 Jan.  2013 Refereed
  • Opt-fMRI analysis for exploring the neuronal connectivity of the hippocampal formation in rats
    Abe Y, Sekino M, Terazono Y, Ohsaki H, Y.Fukazawa, Yawo H, Hisatsune T
    Neurosci Res 74 248-255 Dec.  2012 Refereed
  • Application of an optogenetic byway for perturbing neuronal activity via glial photostimulation
    Sasaki T, Beppu K, Tanaka K, Y.Fukazawa, Shigemoto R, Matsui K
    PNAS 109 20720-20725 Dec.  2012 Refereed
  • Quantitative regional and ultrastructural localization of Cav2.3 subunit of R-type calcium channel in mouse brain
    Parajuli LK, Nakajima C, Kulik A, Matsui K, Schneider S, Shigemoto R, Y.Fukazawa
    J Neurosci 32 13555-13567 Sep.  2012 Refereed
  • Light-evoked somatosensory perception of transgenic rats which express channelrhodopsin-2 in dorsal root ganglion cells
    Ji Z-G, Ito S, Ohta H, Ishizuka T, Y.Fukazawa, Yawo H
    PLoS ONE 7 e32699 Sep.  2012 Refereed
  • Developmental profile of SK2 channel expression and function in CA1 neurons
    Ballesteros-Merino C, Lin M, Wu WW, Ferrandiz-Huertas C, Cabanero MJ, Watanabe M, Y.Fukazawa, Shigemoto R, Maylie J, Adelman JP, Lujan R
    Hippocampus 22 1467-1480 Jun.  2012 Refereed
  • Virus-mediated swapping of zolpidem-insensitive with zolpidem-sensitive GABAA receptors in cortical pyramidal cells
    Sumegi M, Y.Fukazawa, Matsui K, Lorincz A, Eyre MD, Nusser Z, Shigemoto R
    J Physiol 590 1517-1534 Apr.  2012 Refereed
  • Intra-synapse-type and inter-synapse-type relationships between synaptic size and AMPAR expression
    Y.Fukazawa, Shigemoto R
    Current Opinion Neurobiology 22 446-452 Feb.  2012 Refereed
  • Mechanisms underlying signal filtering at a multi-synapse contact
    Budisantoso T, Matsui K, Kamasawa N, Y.Fukazawa, Shigemoto R
    J Neurosci 32 2357-2376 Jan.  2012 Refereed
  • NMDA receptors in hippocampal GABAergic synapses and their role in nitric oxide signaling
    Szabadits E, Cserep C, Sz?nyi A, Y.Fukazawa, Shigemoto R, Watanabe M, Itohara S, Freund T, Nyiri G
    J Neurosci 31 5893-5904 2011 Refereed
  • Quantitative localization of synaptic and extrasynaptic GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica immunolabelling
    Kasugai Y, Swinny JD, Roberts JDB, Dalezios Y, Y.Fukazawa, Sieghart W, Shigemoto R, Somogyi P
    Eu J Neurosci 32 1868-1888 Dec.  2010 Refereed
  • Differential postsynaptic compartments in the laterocapsular division of the central nucleus of amygdale for afferents from the parabrachial nucleus and the basolateral nucleus in the rat
    Dong Y-L, Y.Fukazawa, Wang W, Kamasawa N, Shigemoto R
    J Comp Neurol 518 4771-4791 Dec.  2010 Refereed
  • Subcellular distribution of α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate nucleus
    Parajuli KL, Y.Fukazawa, Watanabe M, Shigemoto R
    J Comp Neurol 518 4362-4374 Nov.  2010 Refereed
  • Direct interaction between orexin neurons activates these neurons through the orexin 2 receptor
    Yamanaka A, Tabuchi S, Tsunematsu T, Y.Fukazawa, Tominaga M
    J Neurosci 30 12642-12652 Sep.  2010 Refereed
  • Cbln1 is a ligand for an orphan glutamate receptor ?2, bidirectional synapse organizer
    Matsuda K, Miura E, Miyazaki T, Kakegawa W, Emi K, Narumi S, Y.Fukazawa, Ito-Ishida A, Kondo T, Shigemoto R, Watanabe M, Yuzaki M
    Science 328 363-368 Apr.  2010 Refereed
  • Tuning of the Zernike phase-plate for visualization of detailed ultrastructure in complex biological specimens
    Fukuda Y, Y.Fukazawa, Danev R, Shigemoto R, Nagayama K
    J Struct Biol 168 476-484 Dec.  2009 Refereed
  • Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter
    Tomita H, Sugano E, Y.Fukazawa, Isago H, Sugiyama Y, Hiroi T, Ishizuka H, Kato M, Hirabayashi M, Shigemoto R, Yawo H, Tamai M
    PLoS One 4 e7679 Nov.  2009 Refereed
  • Crosstalk between GABA(B) and mGlu1α receptors reveals new insight into GPCR signal integration
    Rives ML, Vol C, Y.Fukazawa, Tinel N, Trinquet E, Ayoub MA, Shigemoto R, Pin JP, Prezeau L
    EMBO J 28 2195-2208 Aug.  2009 Refereed
  • Large-conductance calcium-activated potassium channels in Purkinje cell plasma membranes are clustered at sites of hypolemmal microdomains
    Kaufmann WA, Y.Fukazawa, Ferraguti F, Kasugai Y, Shigemoto R, Laake P, Sexton JA, Ruth P, Wietzorrek G, Knaus H-G, Strom JF, Ottersen OP
    J Comp Neurol 515 215-230 Jul.  2009 Refereed
  • Input-specific intrasynaptic arrangements of ionotropic glutamate receptors and their impact on postsynaptic responses
    Tarusawa E, Matsui K, Budisantoso T, Molnar E, Watanabe M, Matsui M, Y.Fukazawa, Shigemoto R
    J Neurosci 29 12896-12908 Jan.  2009 Refereed
  • Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo
    Grinevich V, Kollerker A, Eliava M, Takada N, Takuma H, Y.Fukazawa, Shigemoto R, Kuhl D, Waters J, Seeburg PH, Osten P
    J Neurosci Methods 184 25-36 Jan.  2009 Refereed
  • Numbers, densities and co-localization of AMPA- and NMDA-type glutamate receptors at individual synapses in the superficial spinal dorsal horn of rats
    Antal M, Y.Fukazawa, Eordogh M, Muszil D, Molnar E, Itakura M, Takahashi M, Shigemoto R
    J Neurosci 28 9692-9701 Sep.  2008 Refereed
  • D1-like dopamine receptors selectively block P/Q-type calcium channels to reduce glutamate release onto cholinergic basal forebrain neurons of immature rats
    Momiyama T, Y.Fukazawa
    J Physiol 580 103-117 Apr.  2007 Refereed
  • A CaV2.1 calcium channel mutation rocker reduces the number of postsynaptic AMPA receptors in parallel fiber-Purkinje cell synapses
    Kodama T, Itsukaichi-Nishida Y, Y.Fukazawa, Wakamori M, Miyata M, Molnar E, Mori Y, Shigemoto R, Imoto K
    Eur J Neurosci 24 2993-3007 Dec.  2006 Refereed
  • Differential localization and regulation of stargazing-like protein, γ-8 and stargazing in the plasma membrane of hippocampal and cortical neurons
    Inamura M, Itakura M, Okamoto H, Hoka S, Mizoguchi A, Y.Fukazawa, Shigemoto R, Yamamori S, Takahashi M
    Neurosci Res 55 45-53 May  2006 Refereed
  • Compartment-dependent co-localization of Kir3.2-containing K+ chnannels and GABAB receptors in hippocampal pyramidal cells
    Kulik A, Vida I, Y.Fukazawa, Guetg N, Kasugai Y, Marker C L, Rigato F, Bettler B, Wickman K, Frotscher M, Shigemoto R
    J Neurosci 26 4289-4297 Apr.  2006 Refereed
  • Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by Freeze-fracture replica labeling
    Hagiwara A, Y.Fukazawa, Deguchi-Tawarada M, Ohtsuka T, Shigemoto R
    J Comp Neurol 489 195-216 Aug.  2005 Refereed
  • Two-dimensional neural activity mapping of the entire population of hippocampal CA1 pyramidal cells responding to fear conditioning
    Y.Fukazawa, Inoue K, Ogura A, Inokuchi K
    Neurosci Res 51 417-425 Apr.  2005 Refereed
  • Involvement of growth factors in induction of persistent proliferation of vaginal epithelium of mice exposed neonatally to diethylstilbestrol
    Sato T, Y.Fukazawa, Ohta Y, Iguchi T
    Reprod Toxicol 19 43-51 Nov.  2004 Refereed
  • Immunocytochemical localization of the alpha 1A subunit of the P/Q-type calcium channel in the rat cerebellum
    Kulik A, Nakadate K, Hagiwara A, Y.Fukazawa, Lujan R, Saito H, Suzuki N, Futatsugi A, Mikoshiba K, Frotscher M, Shigemoto R
    Eur J Neurosci Apr.  2004 Refereed
  • Activation of ERK cascade promotes accumulation of Vesl-1S/Homer-1a immunoreactivity at synapses
    Kato A, Y.Fukazawa, Ozawa F, Inokuchi K, Sugiyama H
    Brain Res Mol Brain Res 118 33-44 Sep.  2003 Refereed
  • Hippocampal LTP is accompanied by enhanced actin content within the dendritic spine that is essential for late LTP maintenance in vivo
    Y.Fukazawa, Saitoh Y, Ozawa Y, Ohta Y, Mizuno K, Inokuchi K
    Neuron 38 447-460 May  2003 Refereed
  • Multiple mechanisms are involved inapoptotic cell death in the mouse uterus and vagina after ovariectomy
    Sato T, Y.Fukazawa, Kojima H, Ohta Y, Iguchi T
    Reprod Toxicol 17 289-297 May  2003 Refereed
  • Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins
    Ageta H, Y.Fukazawa, Kato A, Inokuchi K, Sugiyama H
    Brain Res Mol Brain Res 97 186-189 Sep.  2001 Refereed
  • Regulated expression of an actin-associated protein, synaptopodin, during long-term Potentiation
    Yamazaki M, Matsuo R, Ozawa F, Y.Fukazawa, Inokuchi K
    J Neurochem 79 192-199 Sep.  2001 Refereed
  • Cloning and characterization of a novel serine/threonine protein kinase gene expressed predominantly in developing brain
    Nara K, Akasako Y, Y.Fukazawa, Iwashita S, Kataoka M, Nagai Y
    Eur J Biochem 268 2642-2651 May  2001 Refereed
  • Phorbol esters promote postsynaptic accumulation of Vesl-1S/Homer-1a protein
    Kato A, Fukuda T, Fukazawa Y, Isojima Y, Fujitani K, Inokuchi K, Sugiyama H
    Eur J Neurosci 13 1292-1302 Apr.  2001 Refereed
  • Effects of Steroid Hormones and Growth Factors on the Development of the Male Mouse Reproductive Tract In Vitro
    Y.Fukazawa, Iguchi T
    Zool Sci 16 153-160 Feb.  1999 Refereed
  • Mode of cell death in the rat metrial gland during peripartum regression
    Y.Fukazawa, Yamamura Y, Sato T, Iguchi T, Ohta Y
    Anat Rec 252 369-377 Nov.  1998 Refereed
  • Novel members of the Vesl/Homer family of PDZ proteins that bind metabotropic glutamate receptors
    Kato A, Ozawa F, Saito Y, Y.Fukazawa, Sugiyama H, Inokuchi K
    J Biol Chem 273 23969-23975 Sep.  1998 Refereed
  • Apoptosis of pregnancy-dependent mammary tumor and transplantable pregnancy-dependent mammary tumor in mice
    Kojima H, Y.Fukazawa, Sato T, Enari M, Matsuzawa A, Tsunoda S, Nagasawa H, Ohta Y, Iguchi T
    Cancer Lett 110 113-121 Dec.  1997 Refereed
  • Involvement of the TNF-? system and the Fas system in the induction of apoptosis of mouse mammary gland after weaning
    Kojima H, Y.Fukazawa, Sato T, Enari M, Tomooka Y, Matsuzawa A, Ohta A, Iguchi T
    Apoptosis 1 201-208 Dec.  1997 Refereed
  • Effects of estrogenic hormones on early development of Xenopus laevis
    Nishimura N, Y.Fukazawa, Uchiyama H, Iguchi T
    J Exp Zool 278 221-233 Jul.  1997 Refereed
  • Apoptotic cell death during the estrous cycle in the rat uterus and vagina
    Sato T, Y.Fukazawa, Kojima H, Enari M, Iguchi T, Ohta Y
    Anat Rec 248 76-83 May  1997 Refereed
  • Mouse anococcygeus muscle: sexual dimorphism and responsiveness to steroid hormones
    Y.Fukazawa, Iguchi T, Bern HA
    J Endocrinol 152 229-237 Feb.  1997 Refereed
  • Effects of neonatal exposure to diethylstilbestrol and tamoxifen on pelvis and femur in male mice
    Y.Fukazawa, Nobata S, Kato M, Tanaka M, Kobayashi S, Ohta Y, Hayashi Y, Iguchi T
    The Anat Rec 244 416-422 Mar.  1996 Refereed
  • Effects of estrogen on ontogenic expression of progesterone and estrogen receptors in rat uterus
    Ohta Y, Y.Fukazawa, Sato T, Suzuki A, Nishimura N, Iguchi T
    Zool Sci 13 143-149 Feb.  1996 Refereed
  • Apoptosis in adult mouse testis induced by experimental cryptorchidism
    Ohta Y, Nishikawa A, Y.Fukazawa, Urushitani H, Matsuzawa A, Nishina Y, Iguchi T
    Acta Anat 157 195-204 1996 Refereed
  • Effects of sex hormones on oncogene expression in the vagina and on the development of sexual dimorphism of the pelvis and anococcygeus muscle in the mouse
    Iguchi T, Y.Fukazawa, Bern HA
    Env Health Perspect 103 79-82 Sep.  1995 Refereed
  • In vitro secretion of insulin-like growth factor-binding proteins from liver of striped bass, Morone saxatilis
    Y.Fukazawa, Siharath K, Iguchi T, Bern HA
    Gen Comp Endocrinol 99 239-247 Aug.  1995 Refereed
  • Polyovular follicles in mouse ovaries exposed neonatally to diethylstilbestrol in vivo and in vitro
    Y.Fukazawa, Iguchi T, Uesugi Y, Takasugi N
    Biol Reprod 43 478-484 Sep.  1990 Refereed
  • Effect of some hormonally active steroids upon the growth of LNCaP human prostate tumor cells in vitro
    Iguchi T, Y.Fukazawa, Tani N, Sato T, Takasugi N, Shuin T, Kubota Y, Petrow V
    The Cancer Journal 3 184-191 1990 Refereed
  • Morphometric analysis of the development of sexual dimorphism of the mouse pelvis
    Iguchi T, Irisawa S, Y.Fukazawa, Uesugi Y, Takasugi N
    The Anat Rec 224 490-494 Aug.  1989 Refereed
  • Strain differences in the induction of polyovular follicles by neonatal treatment with diethylstilbestrol in mice
    Iguchi T, Ohta Y, Y.Fukazawa, Takasugi N
    Med Sci Res 15 1407-1408 1987 Refereed
  • Opposing Roles of Dopamine Receptor D1-and D2-Expressing Neurons in the Anteromedial Olfactory Tubercle in Acquisition of Place Preference in Mice
    Murata, Koshi;Kinoshita, Tomoki;Fukazawa, Yugo;Kobayashi, Kenta;Yamanaka, Akihiro;Hikida, Takatoshi;Manabe, Hiroyuki;Yamaguchi, Masahiro
    FRONTIERS IN BEHAVIORAL NEUROSCIENCE 13 Mar.  2019
  • Microglial SIRP alpha regulates the emergence of CD11c(+) microglia and demyelination damage in white matter
    Sato-Hashimoto, Miho;Nozu, Tomomi;Toriba, Riho;Horikoshi, Ayano;Akaike, Miho;Kawamoto, Kyoko;Hirose, Ayaka;Hayashi, Yuriko;Nagai, Hiromi;Shimizu, Wakana;Saiki, Ayaka;Ishikawa, Tatsuya;Elhanbly, Ruwaida;Kotani, Takenori;Murata, Yoji;Saito, Yasuyuki;Naruse, Masae;Shibasaki, Koji;Oldenborg, Per-Arne;Jung, Steffen;Matozaki, Takashi;Fukazawa, Yugo;Ohnishi, Hiroshi
    ELIFE 8 Mar.  2019
  • GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice
    Murata, Koshi;Kinoshita, Tomoki;Fukazawa, Yugo;Kobayashi, Kenta;Kobayashi, Kazuto;Miyamichi, Kazunari;Okuno, Hiroyuki;Bito, Haruhiko;Sakurai, Yoshio;Yamaguchi, Masahiro;Mori, Kensaku;Manabe, Hiroyuki
    SCIENTIFIC REPORTS 9 May  2019
  • Differential association of GABAB receptors with their effector ion channels in Purkinje cells.
    Luján Rafael;Aguado Carolina;Ciruela Francisco;Cózar Javier;Kleindienst David;de la Ossa Luis;Bettler Bernhard;Wickman Kevin;Watanabe Masahiko;Shigemoto Ryuichi;Fukazawa Yugo
    Brain structure & function 223(3) 2018
    :Metabotropic GABAB receptors mediate slow inhibitory effects presynaptically and postsynaptically through the modulation of different effector signalling pathways. Here, we analysed the distribution of GABAB receptors using highly sensitive SDS-digested freeze-fracture replica labelling in mouse cerebellar Purkinje cells. Immunoreactivity for GABAB1 was observed on presynaptic and, more abundantly, on postsynaptic compartments, showing both scattered and clustered distribution patterns. Quantitative analysis of immunoparticles revealed a somato-dendritic gradient, with the density of immunoparticles increasing 26-fold from somata to dendritic spines. To understand the spatial relationship of GABAB receptors with two key effector ion channels, the G protein-gated inwardly rectifying K+ (GIRK/Kir3) channel and the voltage-dependent Ca2+ channel, biochemical and immunohistochemical approaches were performed. Co-immunoprecipitation analysis demonstrated that GABAB receptors co-assembled with GIRK and CaV2.1 channels in the cerebellum. Using double-labelling immunoelectron microscopic techniques, co-clustering between GABAB1 and GIRK2 was detected in dendritic spines, whereas they were mainly segregated in the dendritic shafts. In contrast, co-clustering of GABAB1 and CaV2.1 was detected in dendritic shafts but not spines. Presynaptically, although no significant co-clustering of GABAB1 and GIRK2 or CaV2.1 channels was detected, inter-cluster distance for GABAB1 and GIRK2 was significantly smaller in the active zone than in the dendritic shafts, and that for GABAB1 and CaV2.1 was significantly smaller in the active zone than in the dendritic shafts and spines. Thus, GABAB receptors are associated with GIRK and CaV2.1 channels in different subcellular compartments. These data provide a better framework for understanding the different roles played by GABAB receptors and their effector ion channels in the cerebellar network.
  • Optogenetic Activation of Non-Nociceptive Aβ Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats.
    Tashima Ryoichi;Koga Keisuke;Sekine Misuzu;Kanehisa Kensho;Kohro Yuta;Tominaga Keiko;Matsushita Katsuyuki;Tozaki-Saitoh Hidetoshi;Fukazawa Yugo;Inoue Kazuhide;Yawo Hiromu;Furue Hidemasa;Tsuda Makoto
    eNeuro 5(1)
    :Neuropathic pain is caused by peripheral nerve injury (PNI). One hallmark symptom is allodynia (pain caused by normally innocuous stimuli), but its mechanistic underpinning remains elusive. Notably, whether selective stimulation of non-nociceptive primary afferent Aβ fibers indeed evokes neuropathic pain-like sensory and emotional behaviors after PNI is unknown, because of the lack of tools to manipulate Aβ fiber function in awake, freely moving animals. In this study, we used a transgenic rat line that enables stimulation of non-nociceptive Aβ fibers by a light-activated channel (channelrhodopsin-2; ChR2). We found that illuminating light to the plantar skin of these rats with PNI elicited pain-like withdrawal behaviors that were resistant to morphine. Light illumination to the skin of PNI rats increased the number of spinal dorsal horn (SDH) Lamina I neurons positive to activity markers (c-Fos and phosphorylated extracellular signal-regulated protein kinase; pERK). Whole-cell recording revealed that optogenetic Aβ fiber stimulation after PNI caused excitation of Lamina I neurons, which were normally silent by this stimulation. Moreover, illuminating the hindpaw of PNI rats resulted in activation of central amygdaloid neurons and produced an aversion to illumination. Thus, these findings provide the first evidence that optogenetic activation of primary afferent Aβ fibers in PNI rats produces excitation of Lamina I neurons and neuropathic pain-like behaviors that were resistant to morphine treatment. This approach may provide a new path for investigating circuits and behaviors of Aβ fiber-mediated neuropathic allodynia with sensory and emotional aspects after PNI and for discovering novel drugs to treat neuropathic pain.
  • Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter.
    Shinoda Tomoyasu;Nagasaka Arata;Inoue Yasuhiro;Higuchi Ryo;Minami Yoshiaki;Kato Kagayaki;Suzuki Makoto;Kondo Takefumi;Kawaue Takumi;Saito Kanako;Ueno Naoto;Fukazawa Yugo;Nagayama Masaharu;Miura Takashi;Adachi Taiji;Miyata Takaki
    PLoS biology 16(4) 2018
    :Neural progenitor cells (NPCs), which are apicobasally elongated and densely packed in the developing brain, systematically move their nuclei/somata in a cell cycle-dependent manner, called interkinetic nuclear migration (IKNM): apical during G2 and basal during G1. Although intracellular molecular mechanisms of individual IKNM have been explored, how heterogeneous IKNMs are collectively coordinated is unknown. Our quantitative cell-biological and in silico analyses revealed that tissue elasticity mechanically assists an initial step of basalward IKNM. When the soma of an M-phase progenitor cell rounds up using actomyosin within the subapical space, a microzone within 10 μm from the surface, which is compressed and elastic because of the apical surface's contractility, laterally pushes the densely neighboring processes of non-M-phase cells. The pressed processes then recoil centripetally and basally to propel the nuclei/somata of the progenitor's daughter cells. Thus, indirect neighbor-assisted transfer of mechanical energy from mother to daughter helps efficient brain development.
  • Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers.
    Lanjakornsiripan Darin;Pior Baek-Jun;Kawaguchi Daichi;Furutachi Shohei;Tahara Tomoaki;Katsuyama Yu;Suzuki Yutaka;Fukazawa Yugo;Gotoh Yukiko
    Nature communications 9(1) 2018
    :Non-pial neocortical astrocytes have historically been thought to comprise largely a nondiverse population of protoplasmic astrocytes. Here we show that astrocytes of the mouse somatosensory cortex manifest layer-specific morphological and molecular differences. Two- and three-dimensional observations revealed that astrocytes in the different layers possess distinct morphologies as reflected by differences in cell orientation, territorial volume, and arborization. The extent of ensheathment of synaptic clefts by astrocytes in layer II/III was greater than that by those in layer VI. Moreover, differences in gene expression were observed between upper-layer and deep-layer astrocytes. Importantly, layer-specific differences in astrocyte properties were abrogated in reeler and Dab1 conditional knockout mice, in which neuronal layers are disturbed, suggesting that neuronal layers are a prerequisite for the observed morphological and molecular differences of neocortical astrocytes. This study thus demonstrates the existence of layer-specific interactions between neurons and astrocytes, which may underlie their layer-specific functions.
  • Optogenetic Control of Synaptic AMPA Receptor Endocytosis Reveals Roles of LTD in Motor Learning.
    Kakegawa Wataru;Katoh Akira;Narumi Sakae;Miura Eriko;Motohashi Junko;Takahashi Akiyo;Kohda Kazuhisa;Fukazawa Yugo;Yuzaki Michisuke;Matsuda Shinji
    Neuron 99(5) 2018
    :Long-term depression (LTD) of AMPA-type glutamate receptor (AMPA receptor)-mediated synaptic transmission has been proposed as a cellular substrate for learning and memory. Although activity-induced AMPA receptor endocytosis is believed to underlie LTD, it remains largely unclear whether LTD and AMPA receptor endocytosis at specific synapses are causally linked to learning and memory in vivo. Here we developed a new optogenetic tool, termed PhotonSABER, which enabled the temporal, spatial, and cell-type-specific control of AMPA receptor endocytosis at active synapses, while the basal synaptic properties and other forms of synaptic plasticity were unaffected. We found that fiberoptic illumination to Purkinje cells expressing PhotonSABER in vivo inhibited cerebellar motor learning during adaptation of the horizontal optokinetic response and vestibulo-ocular reflex, as well as synaptic AMPA receptor decrease in the flocculus. Our results demonstrate that LTD and AMPA receptor endocytosis at specific neuronal circuits were directly responsible for motor learning in vivo. VIDEO ABSTRACT.
  • SK2 Channels Associate With mGlu1α Receptors and CaV2.1 Channels in Purkinje Cells.
    Luján Rafael;Aguado Carolina;Ciruela Francisco;Arus Xavier Morató;Martín-Belmonte Alejandro;Alfaro-Ruiz Rocío;Martínez-Gómez Jesús;de la Ossa Luis;Watanabe Masahiko;Adelman John P;Shigemoto Ryuichi;Fukazawa Yugo
    Frontiers in cellular neuroscience 12 2018
    :The small-conductance, Ca2+-activated K+ (SK) channel subtype SK2 regulates the spike rate and firing frequency, as well as Ca2+ transients in Purkinje cells (PCs). To understand the molecular basis by which SK2 channels mediate these functions, we analyzed the exact location and densities of SK2 channels along the neuronal surface of the mouse cerebellar PCs using SDS-digested freeze-fracture replica labeling (SDS-FRL) of high sensitivity combined with quantitative analyses. Immunogold particles for SK2 were observed on post- and pre-synaptic compartments showing both scattered and clustered distribution patterns. We found an axo-somato-dendritic gradient of the SK2 particle density increasing 12-fold from soma to dendritic spines. Using two different immunogold approaches, we also found that SK2 immunoparticles were frequently adjacent to, but never overlap with, the postsynaptic density of excitatory synapses in PC spines. Co-immunoprecipitation analysis demonstrated that SK2 channels form macromolecular complexes with two types of proteins that mobilize Ca2+: CaV2.1 channels and mGlu1α receptors in the cerebellum. Freeze-fracture replica double-labeling showed significant co-clustering of particles for SK2 with those for CaV2.1 channels and mGlu1α receptors. SK2 channels were also detected at presynaptic sites, mostly at the presynaptic active zone (AZ), where they are close to CaV2.1 channels, though they are not significantly co-clustered. These data demonstrate that SK2 channels located in different neuronal compartments can associate with distinct proteins mobilizing Ca2+, and suggest that the ultrastructural association of SK2 with CaV2.1 and mGlu1α provides the mechanism that ensures voltage (excitability) regulation by distinct intracellular Ca2+ transients in PCs.
  • Opposing Roles of Dopamine Receptor D1- and D2-Expressing Neurons in the Anteromedial Olfactory Tubercle in Acquisition of Place Preference in Mice.
    Murata Koshi;Kinoshita Tomoki;Fukazawa Yugo;Kobayashi Kenta;Yamanaka Akihiro;Hikida Takatoshi;Manabe Hiroyuki;Yamaguchi Masahiro
    Frontiers in behavioral neuroscience 13 2019
    :Olfaction induces adaptive motivated behaviors. Odors associated with food induce attractive behavior, whereas those associated with dangers induce aversive behavior. We previously reported that learned odor-induced attractive and aversive behaviors accompany activation of the olfactory tubercle (OT) in a domain- and cell type-specific manner. Odor cues associated with a sugar reward induced attractive behavior and c-fos expression in the dopamine receptor D1-expressing neurons (D1 neurons) in the anteromedial OT. In contrast, odor cues associated with electrical shock induced aversive behavior and c-fos expression in the pamine receptor D2-expressing neurons (D2 neurons) in the anteromedial OT, as well as the D1 neurons in the lateral OT. Here, we investigated whether the D1 and D2 neurons in the anteromedial OT play distinct roles in attractive or aversive behaviors, using optogenetic stimulation and real-time place preference (RTPP) tests. Mice expressing ChETA (ChR2/E123T)-enhanced yellow fluorescent protein (EYFP) in the D1 neurons in the anteromedial OT spent a longer time in the photo-stimulation side of the place preference chamber than the control mice expressing EYFP. On the other hand, upon optogenetic stimulation of the D2 neurons in the anteromedial OT, the mice spent a shorter time in the photo-stimulation side than the control mice. Local neural activation in the anteromedial OT during the RTPP tests was confirmed by c-fos mRNA expression. These results suggest that the D1 and D2 neurons in the anteromedial OT play opposing roles in attractive and aversive behaviors, respectively.
  • GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice.
    Murata Koshi;Kinoshita Tomoki;Fukazawa Yugo;Kobayashi Kenta;Kobayashi Kazuto;Miyamichi Kazunari;Okuno Hiroyuki;Bito Haruhiko;Sakurai Yoshio;Yamaguchi Masahiro;Mori Kensaku;Manabe Hiroyuki
    Scientific reports 9(1) 2019
    :Olfaction guides goal-directed behaviours including feeding. To investigate how central olfactory neural circuits control feeding behaviour in mice, we performed retrograde tracing from the lateral hypothalamus (LH), an important feeding centre. We observed a cluster of retrogradely labelled cells distributed in the posteroventral region of the olfactory peduncle. Histochemical analyses revealed that the majority of these retrogradely labelled projection neurons expressed glutamic acid decarboxylase 65/67 (GAD65/67), but not vesicular glutamate transporter 1 (VGluT1). We named this region containing GABAergic projection neurons the ventral olfactory nucleus (VON) to differentiate it from the conventional olfactory peduncle. VON neurons were less immunoreactive for DARPP-32, a striatal neuron marker, compared to neurons in the olfactory tubercle and nucleus accumbens, which distinguished the VON from the ventral striatum. Fluorescent labelling confirmed putative synaptic contacts between VON neurons and olfactory bulb projection neurons. Rabies-virus-mediated trans-synaptic labelling revealed that VON neurons received synaptic inputs from the olfactory bulb, other olfactory cortices, horizontal limb of the diagonal band, and prefrontal cortex. Collectively, these results identify novel GABAergic projection neurons in the olfactory cortex that may integrate olfactory sensory and top-down inputs and send inhibitory output to the LH, which may modulate odour-guided LH-related behaviours.

Books etc

  • Methods in Cell Biology 136 Septins
    Parajuli LK, Ageta-Ishihara N, Ageta H, Fukazawa Y, Kinoshita M
    Methods for immunoblot detection and electron microscopic localization of septin subunits in mammalian nervous systems
    Academic Press Jul.  2016
    978-0-12-803998-4
  • Encyclopedia of Biophysics
    Y.Fukazawa, Fujimoto T
    Electron microscopy of biological membrane.
    Oxford: Academic Press. 2013
  • Encyclopedia of Neuroscience
    Y.Fukazawa, Shigemoto R
    Glutamate receptor organization: ultrastructural insights.
    Oxford: Academic Press. 2009
  • Handbook of Cryo-Preparation Methods for Electron Microscopy.
    Y.Fukazawa, Masugi-Tokita M, Tarusawa E, Hagiwara A, Shigemoto R
     2007

Conference Activities & Talks

  • SBF-SEM assisted quantitative analysis for structural organization of axospinous synapses in normal and disease-model mice
    Yugo Fukazawa
     Feb.  2017
  • Common and unique ultrastructural rules across axospinous synapses in the mouse brain revealed by FIB-SEM imaging
    Ruwaida Elhanbaly, Tatsuya Ishikawa, Yugo Fukazawa
    The 1st ABiS symposium Feb.  2017
  • Common and unique ultrastructural rules of the central excitatory synapses revealed by FIB-SEM
    Ruwaida Elhanbaly, Tatsuya Ishikawa, Yugo Fukazawa
     Dec.  2016
  • Common and unique ultrastructural rules of the central excitatory synapses revealed by FIB-SEM
    Ruwaida Elhanbaly, Tatsuya Ishikawa, Yugo Fukazawa
     Nov.  2016
  • Phosphoinositide responsive Phldb2 regulates synaptic plasticity
    MJ.Xie, H.Yagi, T.Iguchi, Y.Oka, Y.Fukazawa, H.Matsuzaki, K.Iwata, Y.Ishikawa, M.Sato
    The Society for Neuroscience 46th annual meeting Nov.  2016 The Society for Neuroscience
  • Glutamate receptor organization of central synapses at a single synapse level and its remodeling in vivo
    Yugo Fukazawa
     Aug.  2016
  • Quantitative morphological analysis of presynaptic fine structures in the mouse hippocampus by focused ion beam-scanning electron microscopy
    Yugo Fukazawa, Ruwaida Elhanbaly, Tatsuya Ishikawa
    39th Annual Meeting of the Japan Neuroscience Society Jul.  2016
  • Comparison of dendritic spine structures across multiple regions of mouse brain
    Laxmi Kumar Parajuli, Hirohide Iwasaki, Yugo Fukazawa, Shigeo Okabe
    39th Annual Meeting of the Japan Neuroscience Society Jul.  2016
  • Cellular and Subcellular distribution of Na/K-ATPase isoforms in the mouse brain
    Tatsuya Ishikawa, Koshi Murata, Kazuki Kuroda, Yugo Fukazawa
    39th Annual Meeting of the Japan Neuroscience Society Jul.  2016
  • The number and distribution of AMPA receptor channels containing fast kinetic GluA3 and GluA4 subunits are target-cell-dependent at auditory nerve synapses
    María E. Rubio, Ko Matsui, Yugo Fukazawa, Naomi Kamasawa, Harumi Harada, Makoto Itakura, Elek Molnár, Manabu Abe, Kenji Sakimura, Ryuichi Shigemoto
    GRC Conference Jul.  2016
  • Qantitative analysis of synaptic fine structures in the mouse hippocampus by focused ion beam-scanning electron microscopy
    Yugo Fukazawa, Ruwaida Elhambaly, Tatsuya Ishikawa
     Mar.  2016
  • Novel mode of action for interkinetic nuclear migration
    Tomoyasu Shinoda, Arata Nagasaka, Ryo Higuchi, Takashi Miura, Yugo Fukazawa, Kagayaki Kato, Mayumi Okamoto, Yasuhiro Inoue, Yoshiaki Minami, Makoto Suzuki, Masaharu Nagayama, Taiji Adachi, Naoto Ueno, Toshihiko Fujimori, Takaki Miyata
     Mar.  2016
  • FILIP-related molecule binds to NMDA receptor and controls spine maturation and synaptic function of the hippocampal neuron
    Kazuki Kuroda, Hideshi Yagi, Min-Jue Xie, Yugo Fukazawa, Yuichiro Oka, Tokuichi Iguchi, Makoto Sato
    Society for Neuroscience 2015 Oct.  2015
  • CAPS1 stabilizes docking state of SVs in hippocampal CA3-CA1 synapses
    Y Shinoda, C Ishii, Y Fukazawa, T Sadakata, T Iwasato, S Itohara, T Furuichi
    Neuroscience 2015 Oct.  2015
  • Phldb2 regulates the synaptic plasticity
    Min-Jue Xie, Hideshi Yagi, Tokuichi Iguchi, Yuichiro Oka, Kazuki Kuroda, Yugo Fukazawa, Hideo Matsuzaki, Keiko Iwata, Michisuke Yuzaki, Shinji Matsuda, Yasuyuki Ishikawa, Makoto Sato
    38th Annual Meeting of the Japan Neuroscience Society Jul.  2015
  • FILIP-related molecule binds to NMDA receptor and controls spine maturation and synaptic function of the hippocampal neuron
    Kazuki Kuroda, Hideshi Yagi, Min-Jue Xie, Minoru Omi, Yugo Fukazawa, Yuichiro Oka, Tokuichi Iguchi Makoto Sato
    38th Annual Meeting of the Japan Neuroscience Society Jul.  2015
  • Quantitative ultrastructural localization of membrane molecules involved in the formation of resting membrane potential of the neuron
    Yugo Fukazawa
    38th Annual Meeting of the Japan Neuroscience Society Jul.  2015
  • Analysis of septin mutant mice with impaired spatial recognition
    Natsumi Ageta-Ishihara, Hiroki Masuda, Yoshimichi Mano, Akihiko Sawada, Yuuki Yano, Keizo Takao, Tsuyoshi Miyakawa, Ryuichi Shigemoto, Yugo Fukazawa, Makoto Kinoshita
    38th Annual Meeting of the Japan Neuroscience Society Jul.  2015
  • Novel mechanism for Interkinetic Nuclear Migration of neural progenitor cell
    Tomoyasu Shinoda, Arata Nagasaka, Ryo Yugo Fukazawa, Yasuhiro Inoue, Kagayaki Kato, Masaharu Nagayama, Taiji Adachi, Takaki Miyata
    38th Annual Meeting of the Japan Neuroscience Society Jul.  2015
  • CAPS1 associates tethering of SVs on the synaptic plasma membrane
    Yo Shinoda, Chiaki Ishii, Yugo Fukazawa, Tetsushi Sadakata, Shigeyoshi Itohara, Takuji Iwasato, Teiichi Furuichi
    38th Annual Meeting of the Japan Neuroscience Society Jul.  2015
  • Quantitative localization of bio-molecules in the neuronal plasma membrane by immuno-electron microscopy
    Yugo Fukazawa
     Mar.  2015
  • The decrease in CA3 BOLD response after the optic stimulation of the dentate gyrus in ChR2-expressing rats suffered from the radiation exposure
    Yoshifumi Abe, Masaki Sekino, Ryota Imaoka,, Hiroyuki Ohsaki, Yasushi Terazono, Shoji Oda, Hiroshi Mitani,, Y.Fukazawa, Hiromu Yowo, Tatsuhiro Hisatsune
    36th Annual Meeting of the Japan Neuroscience Society Jun.  2013
  • Glutamate Receptor Organization of Central Synapses at The Single Synapse Level and Its Remodeling In Vivo
    Y.Fukazawa
    Global COE 5th International Symposium on Neuro-Tumor Biology and Medicine Feb.  2013
  • Quantitative analysis of synaptic morphology and molecules, and its functional implications
    Y.Fukazawa
    Symposium on “behavior centered high quality research Dec.  2012
  • In situ localization of various lipids in neuronal plasma membranes
    Y.Fukazawa
    Global COE 4th International Symposium on Neuro-Tumor Biology and Medicine Nov.  2012
  • In situ localization of membrane lipids in neuronal plasma membrane
    Kuki T, Ohshiro T, Y.Fukazawa, Matsuzaka K, Yawo h, Muchiake H
    35th Annual Meeting of the Japan Neuroscience Society Sep.  2012
  • Analysis of the high frequency activity during the up state of optogenetically entrained neocortical slow oscillation in the anesthetized rat cortex
    Y.Fukazawa
    35th Annual Meeting of the Japan Neuroscience Society Sep.  2012
  • Optogenic actuation of trigeminal mechanoreceptive neurons from Thy1-ChR2 rat
    Ji Z, Honjoh T, Ishizaka T, Y.Fukazawa, Yawo H
    35th Annual Meeting of the Japan Neuroscience Society Sep.  2012
  • Neural activity and behavior driven by astrocytes
    Matsui K, Sasaki T, Beppu K, Tanaka K, Y.Fukazawa, Shigemoto R
    35th Annual Meeting of the Japan Neuroscience Society Sep.  2012
  • Right-hemispheric dominance of dentate granular cell c-fos expression after spatial exploration in split-brain mice.
    Y.Fukazawa, Ahmed H, Kawakami R, Shigemoto R
    34th Annual Meeting of the Japan Neuroscience Society Sep.  2011
  • Ultrastructural localization analysis of septins in mammalian nervous system
    Kurita H, Y.Fukazawa, Ageta-Ishihara N, Shigemoto R, Kinoshita M
    34th Annual Meeting of the Japan Neuroscience Society Sep.  2011
  • Analysis of functional connectivity among cortical layers during optogenetically induced perturbations
    Kuki T, Matuzaka Y, Y.Fukazawa, Yawo H, Muchiake H
    34th Annual Meeting of the Japan Neuroscience Society Sep.  2011
  • Presynaptic localization of R-type calcium channel in the interpeduncular nucleus
    Parajuli LK, Y.Fukazawa, Shigemoto R
    34th Annual Meeting of the Japan Neuroscience Society Sep.  2011
  • Structural correlate of long-term motor memory formation in cerebeller cortex of mice
    Aziz W, Wang W, Y.Fukazawa, Shigemoto R
    8th IBRO world congress of neuroscience Jul.  2011
  • Right hemisphere dominance of dentate granule cell activation after exploration of novel environment in wild-type and iv mutant mice
    Shigemoto R, Armed H, Kawakami H, Y.Fukazawa
    8th IBRO world congress of neuroscience Jul.  2011
  • GABA(B) receptor activity dinamically regulates expression of Kir3 channels
    Kulik A, Y.Fukazawa, Shigemoto R, Frotscher M, Vida I
    Neuroscience2010 Nov.  2010
  • NMDA receptors in hippocampal GABAergic synapses
    Szabadits E, Sz?nyi A, Cserep C, Y.Fukazawa, Watanabe M, Shigemoto R, Freund TF, Nyiri G
    Neuroscience2010 Nov.  2010
  • Structural changes at parallel fiber-Purkinje cell synapses after long-term adaptation of horizontal optokinetic response in mice
    Shigemoto R, Aziz W, Y.Fukazawa, Wang W, Tarusawa E
    Neuroscience2010 Nov.  2010
  • Orexin neurons are directly and indirectly activated by orexin through the orexin 2 receptor
    Tabuchi S, Tsunematsu T, Y.Fukazawa, Tominaga M, Yamanaka A
    Neuroscience2010 Nov.  2010
  • Exploring functions of the septin cytoskeleton in the formation and remodeling of neuroglial network
    Ageta-Ishihara N, Hagiwara A, Kurita H, Morita T, Tominaga T, Y.Fukazawa, Shigemoto R, Kinoshita M
    33th Annual Meeting of the Japan Neuroscience Society Sep.  2010
  • Regional and subcellular distribution of the α1E subunit of voltage-gated calcium channel in the adult mouse brain
    Parajuli KP, Y.Fukazawa, Kulik A, Shigemoto R
    33th Annual Meeting of the Japan Neuroscience Society Sep.  2010
  • Orexin 2 receptor positively regulates the activity of orexin neurons
    Tabuchi S, Tsunematsu T, Tominaga M, Y.Fukazawa, Yamanaka A
    33th Annual Meeting of the Japan Neuroscience Society Sep.  2010
  • Characterization of DRG neuron subpopulations selectively expressing ChR2 in Thy-1.2 transgenic rat
    Ji Z, Ishizuka T, Y.Fukazawa, Shigemoto R, Yawo H
    33th Annual Meeting of the Japan Neuroscience Society Sep.  2010
  • Presynaptic, postsynaptic, and morphological determinants of signal transmission at the retinogeniculate synapse
    Matsui K, Budisantoso T, Kamasawa N, Y.Fukazawa, Shigemoto R
    33th Annual Meeting of the Japan Neuroscience Society Sep.  2010
  • GABA(B) receptor activity dynamically regulates surface expression of Kir3 channels
    Kulik A, Y.Fukazawa, Shigemoto R, Frotscher M, Vida I
    FENS2010 Jul.  2010
  • Cross-regulation between GABAB and mGlu1a receptors reveals new insight into signal integration between GPCRs
    Prezeau L, Rives ML, Y.Fukazawa, Tinel N, Trinquet E, Ayoub M, Shigemoto R, Pin JP
    FENS2010 Jul.  2010
  • In vivo remodeling of postsynaptic glutamate receptor organization revealed by freeze-fracture replica labeling
    Y.Fukazawa
    Vollum Special Seminar Oct.  2009
  • In vivo remodeling of postsynaptic glutamate receptor organization revealed by freeze-fracture replica labeling.
    Y.Fukazawa, Itakura M, Takahashi T, Saitoh Y, Inokuchi K, Molnar E, Shigemoto R
    Neuroscience 2009 Sep.  2009
  • Compartmentalized distribution of a1G subunit of T-type calcium channel in the dorsal lateral geniculate nucleus of mouse brain
    Parajuli LK, Y.Fukazawa, Watanabe M, Shigemoto R
    Neuroscience 2009 Sep.  2009
  • Structural changes at parallel fiber to Purkinje cells synapses after long-term adaptation of horizontal optokinetic response
    Aziz W, Y.Fukazawa, Tarusawa E, Shigemoto R
    Neuroscience 2009 Sep.  2009
  • Remodeling of postsynaptic glutamate receptor organization in awake rats revealed by freeze-fracture replica labeling
    Y.Fukazawa, Itakura M, Takahashi T, Saitoh Y, Inokuchi K, Molnar E, Shigemoto R
    IUPS2009 Jul.  2009
  • Structural changes at parallel fiber-Purkinje cells synapses after long-term adaptation of horizontal optokinetic response in mice
    Aziz W, Y.Fukazawa, Tarusawa E, Shigemoto R
    IUPS2009 Jul.  2009
  • The enhancement of PSD size and AMPA receptor density in the central amygdala in rat modle of neuropathic pain
    Dong D, Y.Fukazawa, Shigemoto R
    IUPS2009 Jul.  2009
  • Immunohistochemical localization of α1G subunit of T-type calcium channel in the dorsal lateral geniculate nucleus of mouse brain
    Parajuli LK, Y.Fukazawa, Watanabe M, Shigemoto R
    IUPS2009 Jul.  2009
  • Zernike phase contract electron microscopy visualizes detailed structures of vitrified biological specimens
    Fukuda Y, Y.Fukazawa, Danev RS, Shigemoto R, Nagayama K
    IUPS2009 Jul.  2009
  • Remodeling of postsynaptic AMPA receptor localization after induction of long-term potentiation in the hippocampus in vivo
    Y.Fukazawa
     Sep.  2006
  • Remodeling of postsynaptic AMPA receptor localization after induction of long-term potentiation in the hippocampus in vivo
    Y.Fukazawa
     Sep.  2006


Copyright (c) MEDIA FUSION Co.,Ltd. All rights reserved.