MURAKAMI Makoto

FacultySurgery 1
Teacher OrganizationSurgery 1
Education and
 Research Organization
University Hospital
PositionSenior Assistant Professor
Last Updated: 19/11/26 14:45

Researcher Profile & Settings

Name

    MURAKAMI Makoto

Affiliation

  •  Surgery 1 Senior Assistant Professor

Education

  • 1999Fukui Medical School 移植学
  • 1992Fukui Medical School

Academic & Professional Experience

  • 1994-2001 Fukui Medical University, Medical doctor. 2001- Fukui Medical University, Research Assistant.

Research Activities

Published Papers

  • Clinical influence of anastomotic stricture caused by pancreatogastrointestinalstomy following pancreatoduodenectomy
    Murakami, Makoto;Kanji, Katayama;Kato, Shigeru;Fujimoto, Daisuke;Morikawa, Mitsuhiro;Koneri, Kenji;Hirono, Yasuo;Goi, Takanori;Yamaguchi, Akio
    SURGERY TODAY 47(5) 581-586 May  2017
  • Sericin in the isolating solution improves the yield of islets isolated from the pancreas.
    Yokoi Shigehiro;Murakami Makoto;Morikawa Mitsuhiro;Goi Takanori;Yamaguchi Akio;Terada Satoshi
    Cytotechnology 68(6) 2016
    :Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This study aims to improve islet transplantation by increasing the yield of islets from the pancreas, both the number of islets and their size. Therefore, we used a sericin-containing isolating solution. Rat pancreatic islets were isolated by collagenase digestion and hand picking. We refer to islets isolated with or without sericin in the isolation solution as the sericin and control group, respectively. Volume yield, endocrine function, and islet morphology were compared between the groups. Histological distribution of sericin was evaluated by immunofluorescence staining to examine its mechanism of action in pancreatic islets. The pancreatic islet yield in the sericin group was significantly higher than that in the control group. The endocrine function of islets in the sericin group was comparable to that of islets isolated by conventional methods. Sericin adhered to the surface of isolated pancreatic islets and colocalized with E-cadherin, a cell membrane protein, which might explain the cytoprotective effects of sericin. The islet morphology tended to be better preserved in the sericin group. Sericin could prevent cytoarchitectural damage during the isolation and purification process, resulting in increased pancreatic islet yield. This suggests that sericin could contribute to islet therapy by enhancing the stability of islets.
  • Large-cell neuroendocrine carcinoma of the common bile duct: a case report and a review of literature.
    Murakami Makoto;Katayama Kanji;Kato Shigeru;Fujimoto Daisuke;Morikawa Mitsuhiro;Koneri Kenji;Hirono Yasuo;Goi Takanori
    Surgical case reports 2(1) 2016
    BACKGROUND:Large-cell neuroendocrine carcinoma (LCNEC) of the bile duct is extremely rare and is a high-grade type of neuroendocrine tumor with an aggressive clinical course. Here, we report a case of LCNEC of the extrahepatic bile duct.;CASE PRESENTATION:An 80-year-old man presented with severe jaundice. Endoscopic retrograde cholangiography and enhanced computed tomography revealed complete obstruction of the common bile duct (CBD) by a dense tumor measuring 1.5 cm in diameter. Although there were no malignant cells in the biliary brush cytology, we suspected a cholangiocarcinoma and performed extrahepatic bile duct resection. Histologically, the LCNEC occupied most of the places deeper than the stratum submucosum and an adenocarcinoma component, approximately 15%, was present in the mucosa. There were no transitional areas between the two components. Immunohistochemically, the LCNEC cells were reactive for CD56 and synaptophysin and had a high MIB-1 index (72%). The patient died of multiple liver, lung, and peritoneal metastases 3 months after surgery.;CONCLUSIONS:LCNEC of the CBD is particularly rare and has a very poor prognosis. Only five cases have been reported in the literature; therefore, there is no established effective therapy, including surgery, for LCNEC of the CBD at present. An accumulation of additional cases and further studies of multimodal treatment are required in the future to improve the prognosis.
  • Clinical influence of anastomotic stricture caused by pancreatogastrointestinalstomy following pancreatoduodenectomy.
    Murakami Makoto;Kanji Katayama;Kato Shigeru;Fujimoto Daisuke;Morikawa Mitsuhiro;Koneri Kenji;Hirono Yasuo;Goi Takanori;Yamaguchi Akio
    Surgery today 2016
    BACKGROUND AND PURPOSE:Pancreatic fistula after pancreatoduodenectomy (PD) is not uncommon, but few reports describe a stricture after pancreatogastrointestinalstomy. We investigated the clinical influence of anastomotic stricture caused by pancreatogastrointestinalstomy after PD.;METHODS:The subjects of this prospective cohort study were 132 patients who underwent PD or pylorus-preserving PD. We reviewed the relationships between pancreatic duct dilatation of the remnant pancreas and several risk factors. We also compared pancreatic duct dilatation with pancreatic atrophy and analyzed nutrient parameters in the first postoperative year.;RESULTS:Patients with a preoperative pancreatic duct diameter less than 3 mm had a significantly dilated postoperative pancreatic duct diameter (p = 0.0001). The average atrophy rate of the remnant pancreas was 26.3 %, with the lowest atrophy rate (7.3 %) seen in patients without pre- or postoperative pancreatic duct dilation. A normal pancreas in which pancreatic duct dilatation developed postoperatively had a high atrophy rate (34.9 %). Moreover, only patients without pre- or postoperative pancreatic dilatation gained body weight (3.9 %).;CONCLUSION:This study shows a significant correlation between pancreatic atrophy rate and weight loss. Atrophy of the remnant pancreas caused by anastomotic stricture influences the exocrine function of patients after PD. The anastomotic method must be improved to prevent pancreatic duct dilatation and allow for early diagnosis and management of stenotic lesions.

Books etc

  • Hyperthermic Oncology from Bench to Bedside
    Kanji Katayama,Mitsuhiro Morikawa,Kenji Koneri,Makoto Murakami,Yasuo Hirono,Takanori Goi,Akio Yamaguchi
    High-Temperature Hyperthermic Intraperitoneal Chemotherapy(H-HIPEC) with Cytoreductive Surgery for Patients with Peritoneal Metastases of Colorectal Cancer and Appendiceal Pseudomyxoma
    Springer Jun.  2016
    978-981-10-0717-0

Conference Activities & Talks

  • Cell-free and Concentrated Ascites Reinfusion Therapy(CART)for intractable ascites associated with cancerrous ascites
    Kanji Katayama,Yasuo Hirono,Fujiko Kitayama,Mika Hayase,Hiromi Tatsuhira,Akiko Saiki,Yoshikazu Hashimoto,Makoto Murakami, Takanori Goi,Akio yamaguchi
    The 16th PENSA congress-Parenteral and enteral Nutrition Society of Asia- Jul.  2015
  • Multidisciplinary treatment for locally advanced unresectable pancreatic cancer
    Kanji Katayama,Makoto Murakami,Mitsuhiro Morikawa,Kenji Koneri,Yasuo Hirono,Takanori Goi,Akio Yamaguchi
    IAP & JPS & AOPA 2016 in Sendai Aug.  2016
  • HIGH-TEMPERATURE HYPERTHERMIC INTRAPERITONEAL CHEMOTHERAPY AND CYTOREDUCTIVE SURGERY FOR PATIENTS WITH APPENDICEAL PSEUDOMYXOMA
    Kanji Katayama,Mitsuhiro Morikawa,Daisuke Fujimoto,Kenji Koneri,Makoto Murakami,Yasuo Hirono,Takanori Goi,Akio Yamaguchi
    10th international congress on peritoneal surface malignancies Nov.  2016