TERADA Satoshi

FacultyApplied Chemistry and Biotechnology
Teacher OrganizationApplied Chemistry and Biotechnology
Education and
 Research Organization
Faculty of Engineering /Graduate School of Engineering
PositionAssociate Professor
Last Updated: 19/07/23 19:04

Researcher Profile & Settings

Name

    TERADA Satoshi

Affiliation

  •  Applied Chemistry and Biotechnology Associate Professor

Research Activities

Published Papers

  • Real-time Noninvasive Monitoring of UV Light-induced Cell Death by the Deflection of a Probe Beam
    X.Z.Wu, T.Kato, S.Terada
     30 1023-1025 Jan.  2014 Refereed
  • Rakkyo fructan as a cryoprotectant for serum-free cryopreservation of mammalian cells
    A.Ogawa, S.Mizui, Y.Chida, M.Shimizu, S.Terada, T.Ohura, K.Kobayashi, S.Yasukawa, N.Moriyama
     118(1) 101-106 Jan.  2014 Refereed
  • Establishment of a mammalian cell line suitable for industrial production of recombinant protein using mutations induced by high-energy beam radiation
    Y.Chida, K.Takagi, S.Terada
     65(6) 955-965 Dec.  2013 Refereed
  • Rice bran extract affects differentiation of mesenchymal stem cells potency into osteogenic cells
    K.Fukumoto, T.Tsuno, M.Taniguchi, S.Terada
     65(6) 937-943 Dec.  2013 Refereed
  • Effect of the Silk Protein Sericin on Production of Adenovirus-Based Gene Therapy Vectors
    K.Yanagihara, S.Terada, M.Miki, M.Sasaki, H.Yamada
     45 59-64 2006 Refereed
  • Supplementation of Sericin is Effective in Maintenance of Islet Survival And Function Under Serum Free Culture
    A.Ogawa, S.Terada, M.Miki, T. Kimura, Y.Akio, M.Sasaki, H.Yamada
     14 107-112 2006
  • Observation of Individual Cell Behaviors To Analyze Mitogenic Effects of Sericin
    T.Toyosawa, S.Terada, M.Sasaki, H.Yamada, M.Kino-oka
     14 155-161 2006
  • Culture of insulinoma cells on pancreas section
    Y.Saito, S.Terada, T.Takezawa
     14 271-276 2006
  • Rakkyo fructan as a cryoprotectant for serum-free cryopreservation of mammalian cells
    Ogawa, Akiko;Mizui, Shinya;Chida, Yasuhito;Shimizu, Masafumi;Terada, Satoshi;Ohura, Takeshi;Kobayashi, Kyo-ichi;Yasukawa, Saori;Moriyama, Nobuyuki
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING 118(1) 101-106 2014
  • Sericin in the isolating solution improves the yield of islets isolated from the pancreas.
    Yokoi Shigehiro;Murakami Makoto;Morikawa Mitsuhiro;Goi Takanori;Yamaguchi Akio;Terada Satoshi
    Cytotechnology 68(6) 2016
    :Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This study aims to improve islet transplantation by increasing the yield of islets from the pancreas, both the number of islets and their size. Therefore, we used a sericin-containing isolating solution. Rat pancreatic islets were isolated by collagenase digestion and hand picking. We refer to islets isolated with or without sericin in the isolation solution as the sericin and control group, respectively. Volume yield, endocrine function, and islet morphology were compared between the groups. Histological distribution of sericin was evaluated by immunofluorescence staining to examine its mechanism of action in pancreatic islets. The pancreatic islet yield in the sericin group was significantly higher than that in the control group. The endocrine function of islets in the sericin group was comparable to that of islets isolated by conventional methods. Sericin adhered to the surface of isolated pancreatic islets and colocalized with E-cadherin, a cell membrane protein, which might explain the cytoprotective effects of sericin. The islet morphology tended to be better preserved in the sericin group. Sericin could prevent cytoarchitectural damage during the isolation and purification process, resulting in increased pancreatic islet yield. This suggests that sericin could contribute to islet therapy by enhancing the stability of islets.
  • Real-time Noninvasive Monitoring of UV Light-induced Cell Death by the Deflection of a Probe Beam
    Wu, Xing-Zheng;Kato, Tomohisa;Terada, Satoshi
    ANALYTICAL SCIENCES 30(10) 1023-1025 2014
  • Real-time Noninvasive Monitoring of UV Light-induced Cell Death by the Deflection of a Probe Beam
    WU Xing-Zheng;KATO Tomohisa;TERADA Satoshi
    Analytical Sciences 30(10) 1023-1025 2014
    Cell death and its deregulation characterize numerous human diseases. Here, we report on real-time noninvasive monitoring of UV light-induced cell death by the deflection of a probe beam. UV light of 330 – 370 nm from a high-pressure Hg lamp illuminated cultured HepG2 cells, and at the same time a probe beam from a diode laser was passed through a vicinity of the HepG2 cell. The deflection signal of the probe beam, which was induced by changes of the concentration gradients in processes of the active materials movements across the cell membrane, was monitored. It was found that the deflection signals changed greatly after UV illumination, suggesting that the materials movements across the cell membrane were greatly affected by the UV illumination. After UV illumination of about 5400 – 7400 s at a light power of 0.028 W/cm2, the deflection signals became little changed with time, suggesting that the living cells had been killed by the UV illumination. This conclusion agreed well with cell viability determinations of the traditional trypan blue method.
  • Microbiome Analysis of Biofilms of Silver Nanoparticle-Dispersed Silane-Based Coated Carbon Steel Using a Next-Generation Sequencing Technique.
    Ogawa Akiko;Takakura Keito;Sano Katsuhiko;Kanematsu Hideyuki;Yamano Takehiko;Saishin Toshikazu;Terada Satoshi
    Antibiotics (Basel, Switzerland) 7(4) 2018
    :Previously, we demonstrated that silver nanoparticle-dispersed silane-based coating could inhibit biofilm formation in conditions where seawater was used as a bacterial source and circulated in a closed laboratory biofilm reactor. However, it is still unclear whether the microbiome of a biofilm of silver nanoparticle-dispersed silane-based coating samples (Ag) differs from that of a biofilm of non-dispersed silane-based coating samples (Non-Ag). This study aimed to perform a microbiome analysis of the biofilms grown on the aforementioned coatings using a next-generation sequencing (NGS) technique. For this, a biofilm formation test was conducted by allowing seawater to flow through a closed laboratory biofilm reactor; subsequently, DNAs extracted from the biofilms of Ag and Non-Ag were used to prepare 16S rRNA amplicon libraries to analyze the microbiomes by NGS. Results of the operational taxonomy unit indicated that the biofilms of Non-Ag and Ag comprised one and no phyla of archaea, respectively, whereas Proteobacteria was the dominant phylum for both biofilms. Additionally, in both biofilms, Non-Ag and Ag, Marinomonas was the primary bacterial group involved in early stage biofilm formation, whereas Anaerospora was primarily involved in late-stage biofilm formation. These results indicate that silver nanoparticles will be unrelated to the bacterial composition of biofilms on the surface of silane-based coatings, while they control biofilm formation there.

Conference Activities & Talks

  • Application of Silane-Based Coating to Biomaterials for Inhibiting Biofilm Induced Infection
    A. Ogawa, A. Tahori, M. Yano, R. Hirobe, S. Terada, D. Kuroda,K. Sano,H.Kanematsu
    31st International Meeting of Japanese Association for Animal Cell Technology Nov.  2018
  • Protective Effect of Sericin Peptide on Mammalian Cell Survival
    Satoshi Terada, Satoshi Hosomi, Kyohei Kuriyama
    International Meeting of Japanese Association of Animal Cell Technology Nov.  2018
  • Silk Protein Sericin as Bio-Active Supplement for Mammalian Culture
    S. Terada
    International Meeting of the MSBMB Aug.  2017
  • "Silk Protein Sericin Peptide as Growth Factor in Mammalian Cell Culture
    S. Terada, N. Sindoh, M. Sasaki, J. Takahashi
    European Society of Animal Cell Technology May  2017
  • 安価な新規増殖促進因子を用いた動物細胞の培養技術
    寺田 聡
     Nov.  2007
  • 福井特産農作物から得られる多糖を用いた細胞凍結液の開発
    寺田 聡
     Nov.  2007
  • 単一細胞の非侵襲的なビーム偏向測定及び化学試薬の細胞への毒性の研究
    加藤 智久, 寺田 聡, 呉 行正
     Sep.  2007
  • 絹蛋白セリシン・ラッキョウフルクタンを利用した細胞培養によるバイオ医薬品生産
    寺田 聡
     Sep.  2007
  • 植物に由来する、動物細胞培養の新規添加因子フルクタン
    東屋 秀治, 寺田 聡, 小林 恭一
     Sep.  2007
  • 動物細胞に対するセリシンの増殖促進機構の解析
    柳原 佳奈, 斉藤 卓也, 三木 正雄, 小川 亜希子, 佐々木 真宏, 山田 英幸, 寺田 聡
     Sep.  2007