TAKEUCHI Ayako

FacultyMorphological and Physiological Sciences, Integrative Systems Physiology
Teacher OrganizationMorphological and Physiological Sciences
Education and
 Research Organization
Faculty of Medical Sciences, School of Medicine
PositionAssociate Professor
Last Updated: 19/09/12 15:15

Researcher Profile & Settings

Name

    TAKEUCHI Ayako

Affiliation

  •  Morphological and Physiological Sciences, Integrative Systems Physiology Associate Professor

Education

  • Apr. 2000Mar. 2003Kyoto University 医療薬科学
  • Apr. 1998Mar. 2000Kyoto University 医療薬科学
  • Apr. 1994Mar. 1998Kyoto University Faculty of Pharmaceutical Sciences

Degree

  • Ph.D.

Academic & Professional Experience

  • Apr. 2017University of Fukui Department of Integrative and Systems Physiology, Faculty of Medical Sciences Associate Professor
  • Sep. 2013Department of Integrative Physiology, Faculty of Medical Sciences, University of Fukui Assistant Professor
  • Apr. 2008Mar. 2009The Rockefeller University Laboratory of Cardiac/Membrane Physiology Research Associate
  • Apr. 2007Aug. 2013Department of Physiology and Biophysics, Kyoto University Graduate School of Medicine Assistant Professor
  • Apr. 2007Mar. 2008The Rockefeller University Laboratory of Cardiac/Membrane Physiology Postdoctoral Associate
  • Dec. 2005Mar. 2007Department of Physiology and Biophysics, Kyoto University Graduate School of Medicine Assistant Professor
  • Aug. 2005Nov. 2005Department of Physiology and Biophysics, Kyoto University Graduate School of Medicine Research Associate
  • Apr. 2004Jul. 2005Cell/Biodynamics Simulation Project Kyoto University Postdoctoral Fellow
  • Apr. 2003Mar. 2004Faculty of Pharmaceutical Sciences, Kyoto University Research Fellow of the Japan Society for the Promotion of Science PD
  • Apr. 2002Mar. 2003Faculty of Pharmaceutical Sciences, Kyoto University Japan Society for the Promotion of Science Research Fellow DC2

Association Memberships

    Society of General Physiologist member,Japanese Heart Rhythm Society member,Physiological Society of Japan councilor,Biophysical Society member,The Pharmaceutical Society of Japan member,

Research Activities

Published Papers

  • Uncovering the arrhythmogenic potential of TRPM4 activation in atrial-derived HL-1 cells using novel recording and numerical approaches
    Hu Y, Duan Y, Takeuchi A, Hai-Kurahara L, Ichikawa J, Hiraishi K, Numata T, Ohara H, Iribe G, Nakaya M, Mori MX, Matsuoka S, Ma G, Inoue R
    Cardiovascular Research 113(10) 1243-1255 Aug.  2017 Refereed
  • Functional analysis of rat renal organic anion transporter OAT-K1: bidirectional methotrexate transport in apical membrane
    Masuda S, Takeuchi A, Saito H, Hashimoto Y, Inui K
    FEBS Lett 459(1) 128-132 1999 Refereed
  • Patient-specific human induced pluripotent stem cell model assessed with electrical pacing validates S107 as a potential therapeutic agent for catecholaminergic polymorphic ventricular tachycardia
    Sasaki K, Makiyama T, Yoshida Y, Wuriyanghai Y, Kamakura T, Nishiuchi S, Hayano M, Harita T, Yamamoto Y, Kohjitani H, Hirose S, Chen J, Itoh H, Kawamura M, Ohno S, Takeuchi A, Matsuoka S, Miura M, Sumitomo N, Horie M, Yamanaka S, KimuraT
    PLoS One 11(10) e0164795 2016 Refereed
  • A simulation study on the constancy of cardiac energy metabolites during workload transition
    Saito R, Takeuchi A, Himeno Y, Inagaki N, Matsuoka S
    The Journal of Physiology 594(23) 6929-6945 2016 Refereed
  • Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage
    Sakamaki K, Ishii TM, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Jung SK, Matsuoka S, Koyamada K, Sawasaki T, Nagai T, Ueno N
    Biochimica Biophysica Acta 1863(11) 2766-2783 2016 Refereed
  • Physiome study on the Ca2+ crosstalk between mitochondria-sarcoplasmic/endoplasmic reticulum
    Takeuchi A
    Membrane 41(5) 215-220 2016 Refereed
  • Roles of the mitochondrial Na+-Ca2+ exchanger, NCLX, in B lymphocyte chemotaxis
    Kim B, Takeuchi A, Hikida M, Matsuoka S
    Scientific Reports 6 28378 2016 Refereed
  • The destiny of Ca2+ released by mitochondria
    Takeuchi, Ayako;Kim, Bongju;Matsuoka, Satoshi
    JOURNAL OF PHYSIOLOGICAL SCIENCES 65(1) 11-24 Jan.  2015 Refereed
  • Role of the Mitochondrial Na+-Ca2+Exchanger, NCLX, in the Generation of Cardiac Automaticity
    Takeuchi Ayako;Matsuoka Satoshi
    Japanese Journal of Electrocardiology 34(2) 69-81 2014 Refereed
    Mitochondria are known as ATP-producing factories as well as Ca2+ stores. Mitochondrial Ca2+ (Ca2+m) changes dynamically, and this change is mainly balanced by an influx via a Ca2+ uniporter (MCU) and an efflux via a Na+-Ca2+ exchanger (NCLX). It has been suggested that Ca2+m is involved in regulating mitochondrial energy metabolism. However, little is known about the roles of NCLX in cardiomyocytes, where repetitive Ca2+ transients occur and where a huge amount of ATP is generated and utilized. To clarify its roles, we carried out a study combining NCLX knockdown in the spontaneously beating atrial myocyte-derived cell line HL-1 and mathematical simulations. NCLX knockdown using siRNA resulted in -50% reduction of its mRNA and protein expressions, attenuating Ca2+m efflux and increasing Ca2+m content. Cycle length of spontaneous Ca2+ oscillation and action potential generation were markedly prolonged. The upstrokes of action potential and Ca2+ transient were slower, and sarcoplasmic reticulum (SR) Ca2+ content and reuptake were reduced by knocking down NCLX. Analysis of a mathematical model of HL-1 demonstrated that a substantial amount of Ca2+ is transferred either directly or indirectly via NCLX from mitochondria to SR and that blocking NCLX reduces the SR Ca2+ content to slow SR Ca2+ leak, which triggers automaticity. We propose that Ca2+m dynamics via NCLX is involved in the generation of abnormal automaticity and arrhythmia.
  • Identification of chemicals inducing cardiomyocyte proliferation in developmental stage-specific manner with pluripotent stem cells
    Uosaki H, Magadum A, Seo K, Fukushima H, Takeuchi A, Nakagawa Y, Moyes KW, Narazaki G, Kuwahara K, Laflamme M, Matsuoka S, Nakatsuji N, Nakao K, Kwon C, Kass DA, Engel FB, Yamashita JK
    Circulation-Cardiovascular Genetics 6(6) 624-33 Dec.  2013 Refereed
  • The mitochondrial Na+-Ca2+ exchanger, NCLX, regulates automaticity of HL-1 cardiomyocytes
    Takeuchi A, Kim B, Matsuoka S
    Scientific Reports 3 2766 Sep.  2013 Refereed
  • Mitochondria Na+-Ca2+ Exchange in Cardiomyocytes and Lymphocytes
    Kim B, Takeuchi A, Koga O, Hikida M, Matsuoka S
    Advances in Experimental Medicine and Biology 961 193-201 2013 Refereed
  • Pivotal role of mitochondrial Na+?Ca2+ exchange in antigen receptor mediated Ca2+ signalling in DT40 and A20 B lymphocytes
    Takeuchi A, Kim B, Koga O, Hikida M, Matsuoka S
    The Journal of Physiology 590(3) 459-474 2012 Refereed
  • NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle
    Hattori H, Takeshita D, Takeuchi A, Kim B, Shibata M, Matsuoka S, Obata K, Mitsuyama S, Zhang GX, Takaki M
    Biochemical and Biophysical Research Communications 419(2) 431-435 2012 Refereed
  • Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression
    Uosaki H, Fukushima H, Takeuchi A, Matsuoka S, Nakatsuji N, Yamanaka S, Yamashita JK
    PLoS One 6(8) e23657 2011 Refereed
  • Impairment of Ubiquitin-Proteasome System by E334K cMyBPC Modifies Channel Proteins, Leading to Electrophysiological Dysfunction
    Bahrudin U, Morikawa K, Takeuchi A, Kurata Y, Miake J, Mizuta E, Adachi K, Higaki K, Yamamoto Y, Shirayoshi Y, Yoshida A, Kato M, Yamamoto K, Nanba E, Morisaki H, Morisaki T, Matsuoka S, Ninomiya H, Hisatome I
    Journal of Molecular Biology 413(4) 857-878 2011 Refereed
  • Peering into an ATPase ion pump with single-channel recordings
    Takeuchi A, Gadsby DC, Artigas P, Reyes N
    Philosophical Transactions of the Royal Society B 364(1514) 229-238 2009 Refereed
  • Increased O2 consumption in excitation-contraction coupling in hypertrophied rat heart slices related to increased Na+-Ca2+ exchange activity
    Shimizu J, Yamashita D, Misawa H, Tohne K, Matsuoka S, Kim B, Takeuchi A, Nakajima-Takenaka C, Takaki M
    The Journal of Physiological Sciences 59 63-74 2009 Refereed
  • Visualizing the mapped ion pathway through the Na,K-ATPase pump
    Takeuchi A, Reyes N, Artigas P, Gadsby DC
    Channels 3(6) 383-386 2009 Refereed
  • Simulation analysis of intracellular Na+ and Cl- homeostasis during beta1-adrenergic stimulation of cardiac myocyte
    Kuzumoto M, Takeuchi A, Nakai H, Oka C, Noma, Matsuoka S
    Progress in Biophysics and Molecular Biology 96 171-186 2008 Refereed
  • The ion pathway through the opened Na+,K+-ATPase pump
    Takeuchi A, Reyes N, Artigas P, Gadsby DC
    Nature 456(7220) 413-416 2008 Refereed
  • Role of Ca2+ transporters and channels in the cardiac cell volume regulation
    Takeuchi A, Tatsumi S, Sarai N, Terashima K, Matsuoka S, Noma A
    Annals of the New York Academy of Sciences 1099 377-382 2007 Refereed
  • Cl- homeostasis and volume regulation of the cardiac cell
    Terashima K, Takeuchi A, Sarai N, Matsuoka S, Shim EB, Leem CH, Noma A
    Philos Trans A Math Phys Eng Sci 364(1842) 1245-1265 2006 Refereed
  • Ionic mechanisms of cardiac cell swelling induced by blocking Na+/K+ pump as revealed by experiments and simulation
    Takeuchi A, Tatsumi S, Sarai N, Terashima K, Matsuoka S, Noma A
    The Journal of General Physiology 128(5) 495-507 2006 Refereed
  • Gene expression variance based on random sequencing in rat remnant kidney
    Horiba N, Masuda S, Takeuchi A, Saito H, Okuda M, Inui K
    Kidney Int 66(1) 29-45 2004 Refereed
  • Cloning and characterization of a novel Na+-dependent glucose transporter (NaGLT1) in rat kidney
    Horiba N, Masuda S, Takeuchi A, Takeuchi D, Okuda M, Inui K
    J Biol Chem 278(17) 14669-14676 2003 Refereed
  • Decreased activity of basolateral organic ion transporters in hyperuricemic rat kidney: roles of organic ion transporters, rOAT1, rOAT3 and rOCT2
    Habu Y, Yano I, Takeuchi A, Saito H, Okuda M, Fukatsu A, Inui K
    Biochem Pharmacol 66(6) 1107-1114 2003 Refereed
  • Decreased function of genetic variants, Pro283Leu and Arg287Gly, in human organic cation transporter hOCT1
    Takeuchi A, Motohashi H, Okuda M, Inui K
    Drug Metab Pharmacokinet 18(6) 409-412 2003 Refereed
  • Role of kidney-specific organic anion transporters in the urinary excretion of methotrexate
    Takeuchi A, Masuda S, Saito H, Doi T, Inui K
    Kidney Int 60(3) 1058-1068 2001 Refereed
  • Multispecific substrate recognition of kidney-specific organic anion transporters OAT-K1 and OAT-K2
    Takeuchi A, Masuda S, Saito H, Abe T, Inui K
    J Pharmacol Exp Ther 299(1) 261-267 2001 Refereed
  • Trans-stimulation effects of folic acid derivatives on methotrexate transport by rat renal organic anion transporter, OAT-K1
    Takeuchi A, Masuda S, Saito H, Hashimoto Y, Inui K
    J Pharmacol Exp Ther 293(3) 1034-1039 2000 Refereed
  • Cloning and functional characteristics of a new multispecific organic anion transporter, OAT-K2, in rat kidney
    Masuda S, Ibaramoto K, Takeuchi A, Saito H, Hashimoto Y, Inui K
    Mol Pharmacol 55(4) 743-752 1999 Refereed

Books etc

  • Patch Clamp Techniques-From beginning to advanced protocols-
    Matsuoka S, Takeuchi A
    Giant Patch and Macro Patch
    Springer Protocols 2012
  • SYSTEM BIOENERGETICS Energy for life
    Matsuoka S, Jo H, Saito R, Takeuchi A, Noma A
    Modeling energetics of ion transport, membrane sensing and system biology of the heart
    WILEY-VCH 2007
  • Control and Diseases of Sodium Dependent Transport Proteins and Ion Channels
    Masuda S, Takeuchi A, Saito H, Hashimoto Y, Inui K
    Tubular localization and drug recognition of organic anion transporters OAT-K1 and OAT-K2
    Elsevier Science B. V. 2000

Conference Activities & Talks

  • Evaluation of effects of empagliflozin on mouse ventricular myocytes
    Hinako Suzuki, Takuma Yoshizawa, Shunsuke Aoki, Saki Watanabe, Yukari Takeda, Ayako Takeuchi and Satoshi Matsuoka
    9th FAOPS congress Mar.  2019
  • The mitochondrial Na+-Ca2+ exchanger is involved in automaticity of murine sinoatrial nodal cells
    Yukari Takeda, Ayako Takeuchi, Satoshi Matsuoka
    9th FAOPS congress Mar.  2019
  • Roles of mitochondrial Ca2+ channels/transporters in cellular functions
    Ayako Takeuchi, Satoshi Matsuoka
    The 49th NIPS International Symposium Ion channels: looking back, seeing ahead Dec.  2018
  • Frequency-dependences of action potential and Ca2+ transient and roles of mitochondrial Na+/Ca2+ exchange in mice ventricular myocytes
    Yukari Takeda, Misaki Tsukioka, Shino Fujisawa, Moe Fujisawa, Yousuke Shimizu, Erika Iwai, Rena Horie, Saki Matsunaka, Ayako Takeuchi and Satoshi Matsuoka
    2017 Cardiac Physiome Workshop Nov.  2017 Cardiac Physiome Society
  • Dysregulation of a potassium channel, THIK-1, targeted by the caspase accelerates cell shrinkage during apoptosis
    Kazuhiro Sakamaki, Toshiya Sakata, Ayako Takeuchi, Chiyo Takagi, Hajime Shinoda, Akiko Saito, Takeharu Nagai, Satoshi Matsuoka, Naoto Ueno
     Dec.  2017 The Molecular Biology Society of Japan
  • Intracellular localization of mitochondrial Na+-Ca2+ exchanger NCLX in mice ventricular myocytes
    Ayako Takeuchi, Satoshi Matsuoka
    62nd Biophysical Meeting Feb.  2018 Biophysical Society
  • A theoretical study on the roles of Ca2+ in the energy metabolite stability during cardiac workload transition
    Takeuchi A, Saito R, Himeno Y, Matsuoka S
    61st Biophysical Meeting Feb.  2017
  • A simulation study on roles of Ca2+ in constancy of cardiac energy metabolites during workload transition
    Takeuchi A, Saito R, Himeno Y, Matsuoka S
    2016 Cardiac Physiome Workshop Aug.  2016
  • Simulation analysis of automaticity of sinoatrial node myocytes
    Takeuchi A, Matsuoka S
    Society of Mathematical Biology 2015 conference Jun.  2015
  • Contribution of mitochondria to the generation of automaticity of sinoatrial node cells; a simulation study
    Takeuchi A, Horiguchi K, Iino S, Fukazawa Y, Matsuoka S
     Sep.  2015
  • HD physiological analysis of mechanisms underlying generation of cardiac automaticity via mitochondria
    Takeuchi A, Matsuoka S
    Grant-in-Aid for Scientific Research on Innovative Area “HD Physiology” The Final HD Physiology Symposium Mar.  2015
  • Mitochondrial Na-Ca exchanger NCLX-mediated mitochondria-sarcoplasmic reticulum Ca crosstalk and cardiomyocyte automaticity
    Takeuchi A, Matsuoka S
    The 91st Annual Meeting of the Physiological Society of Japan Mar.  2014
  • Mitochondria-endoplasmic/sarcoplasmic reticulum Ca crosstalk and cellular function
    Takeuchi A, Kim B, Matsuoka S
    The 91st Annual Meeting of the Physiological Society of Japan Mar.  2014
  • Contribution of mitochondrial Na+-Ca2+ Exchanger, NCLX, in making the cardiac rhythmicity. A combination study of experiments and simulations
    Takeuchi A, Kim B, Matsuoka S
    The 2nd HD Physiology International Symposium: Multi-Level Systems Biology Jun.  2013
  • Theoretical analysis of mitochondrial NCX-mediated regulation of automaticity in sinoatrial node cells
    Takeuchi A, Kim B, Matsuoka S
    The 2nd HD Physiology International Symposium: Multi-Level Systems Biology Jun.  2013
  • Simulation analyses of the role of NCLX, a mitochondrial Na+-Ca2+ exchanger, on the regulation of automaticity in sinoatrial node cells
    Takeuchi A, Matsuoka S
    2013 Cardiac Physiome Workshop Jan.  2013
  • Mitochondria-sarcoplasmic reticulum Ca2+ communication on the rhythmicity of sinoatrial node cells
    Matsuoka S, Takeuchi A
    2013 Cardiac Physiome Workshop Jan.  2013

Awards & Honors

  •  Cardiac Physiome Cardiac Physiome Workshop 2012 Poster Prize Winner2012