KONISHI Yoshiyuki

FacultyApplied Chemistry and Biotechnology
Teacher OrganizationApplied Chemistry and Biotechnology
Education and
 Research Organization
Faculty of Engineering /Graduate School of Engineering
Last Updated: 19/12/24 10:09

Researcher Profile & Settings


    KONISHI Yoshiyuki


  •  Applied Chemistry and Biotechnology Professor


  • Apr. 1997Mar. 2000Chiba University
  • Apr. 1995Mar. 1997Chiba University
  • Apr. 1991Mar. 1995Chiba University

Academic & Professional Experience

  • Apr. 2011Graduate School of Engineering Associate Professor
  • Nov. 2008Mar. 2011Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine Associate Professor
  • Apr. 2006Jan. 2008Mitsubishi Kagaku Institute of Life Sciences Senior Researcher
  • Jul. 2004Mar. 2006Center for Molecular Medicine, Jichi Medical School Assistant Professor
  • Jul. 2000Jun. 2004Department of Pathology, Harvard Medical School Research Fellow
  • Mar. 2000Jun. 2000Faculty of Science, Chiba University Research Associate
  • Apr. 1997Mar. 2000Japan Society for the Promotion of Science Research Fellow (DC1)

Research Activities

Published Papers

  • Inhibition of glycogen synthase kinase-3 reduces extension of the axonal leading process by destabilizing microtubules in cerebellar granule neurons.
    Inami Y, Omura M, Kubota K, Konishi Y.
    Brain Res. 1690 51-60 Apr.  2018 Refereed
  • Differential retraction of axonal arbor terminals mediated by microtubule and kinesin motor
    Ikeno T, Konishi Y
    Commun. Integr. Biol. e1288771 Feb.  2017 Refereed
  • Mechanisms of differential branch growth control in the single axonal arbor
    Konishi Y
    Forma 32 S25-S28 Jan.  2017 Refereed
  • Study of local intracellular signals regulating axonal morphogenesis using a microfluidic device
    Uryu D, Tamaru T, Suzuki A, Sakai R, Konishi Y
    Sci. Technol. Adv. Mater. 17(1) 691-697 Oct.  2016 Refereed
  • Kinesin-1 sorting in axons controls the differential retraction of arbor terminals.
    Seno T, Ikeno T, Mennya K, Kurishita M, Sakae N, Sato M, Takada H, Konishi Y
     29 3499-3510 Sep.  2016 Refereed
  • Ttll9-/- mice sperm flagella show shortening of doublet 7, reduction of doublet 5 polyglutamylation and a stall in beating
    Konno A, Ikegami K, Konishi Y, Yang HJ, Abe M, Yamazaki M, Sakimura K, Yao I, Shiba K, Inaba K, Setou M
    J Cell Sci. 129 2757-2766 Jul.  2016 Refereed
  • DBZ regulates cortical cell positioning and neurite development by sustaining the anterograde transport of Lis1 and DISC1 through control of Ndel1 dual-phosphorylation
    M.Okamoto, T.Iguchi, T.Hattori, S.Matsuzaki, Y.Koyama, M.Taniguchi, M.Komada, M-J.Xie, H.Yagi, S.Simizu, Y.Konishi, M.Omi, T.Yoshimi, T.Tachibana, S.Fujieda, T.Katayama, A.Ito, S.Hirotsune, M.Tohyama, M.Sato
    J. Neurosci. 35(7) 2942-2958 Feb.  2015 Refereed
  • Cellular mechanisms for the axonal pattern formation: Initiation and branch morphogenesis
    Y.Konishi (corresponding author)
     29 51-54 Sep.  2014 Refereed
  • A low-density culture method of cerebellar granule neurons with paracrine support applicable for the study of neuronal morphogenesis
    K Kubota, T Seno, Y Konishi
    Brain Res. 1539 15-23 2013 Refereed
  • Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics
    Yang HJ, Sugiura Y, Ikegami K, Konishi Y, Setou M
     287(8) 5290-5300 2012 Refereed
  • Imaging mass spectrometry revealed the production of lyso-phosphatidylcholine in the injured ischemic rat brain
    Koizumi S, Yamamoto S, Hayasaka T, Konishi Y, Yamaguchi-Okada M, Goto-Inoue N, Sugiura Y, Setou M, Namba H
    Neuroscience 168 219-225 2010 Refereed
  • Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs)
    Kimura Y, Kurabe N, Ikegami K, Tsutsumi K, Konishi Y, Kaplan OI, Kunitomo H, Iino Y, Blacque OE, Setou M
    J Biol Chem 285 22936-22941 2010 Refereed
  • Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry
    Sugiura Y, Konishi Y, Zaima N, Kajihara S, Nakanishi H, Taguchi R, Setou M
    J Lipid Res 50 1776-1788 2009 Refereed
  • Tubulin Tyrosination Navigates the Kinesin-1 Motor Domain to Axons
    Konishi Y, Setou M
    Nat Neurosci. 12 559-567 2009 Refereed
  • Enhancement of Trk Signaling Pathways by the Cholestane Amide Conjugate MCC-257
    Yamada MK, Konishi Y, Kakinoki B, Ikegami K, Setou M
    J Pharmacol Sci. 108 131-134 2008 Refereed
  • Imaging mass spectrometry technology and application on ganglioside study; visualization of age-dependent accumulation of C20-ganglioside molecular species in the mouse hippocampus
    Sugiura Y, Shimma S, Konishi Y, Yamada MK, Setou M
    PLoS ONE 3 e3232 2008 Refereed
  • Transmembrane and ubiquitin-like domain-containing protein 1 (Tmub1/HOPS) facilitates surface expression of GluR2-containing AMPA receptors
    Yang H, Takagi H, Konishi Y, Ageta H, Ikegami K, Yao I, Sato S, Hatanaka K, Inokuchi K, Seog DH, Setou M
    PLoS ONE 3 e2809 2008 Refereed
  • Activation of FOXO1 by Cdk1 in cycling cells and postmitotic neurons
    Yuan Z, Becker EB, Merlo P, Yamada T, DiBacco S, Konishi Y, Schaefer EM, Bonni A
    Science 319 1665-1668 2008 Refereed
  • TGFbeta-Smad2 signaling regulates the Cdh1-APC/SnoN pathway of axonal morphogenesis
    Stegmuller J, Huynh MA, Yuan Z, Konishi Y, Bonni A
    J Neurosci. 28 1961-1969 2008 Refereed
  • Six1 and Six4 promote survival of sensory neurons during early trigeminal gangliogenesis
    Konishi Y, Ikeda K, Iwakura Y, Kawakami K
    Brain Res. 1116 93-102 2006 Refereed
  • Cell-intrinsic regulation of axonal morphogenesis by the Cdh1-APC target SnoN
    Stegmuller J, Konishi Y, Huynh MA, Yuan Z, Dibacco S, Bonni A
    Neuron 50 389-400 2006 Refereed
  • Phosphorylation of BAD at Ser-128 during mitosis and paclitaxel-induced apoptosis
    Berndtsson M, Konishi Y, Bonni A, Hagg M, Shoshan M, Linder S, Havelka AM
    FEBS Lett. 579 3090-3094 2005 Refereed
  • Cdh1-APC controls axonal growth and patterning in the mammalian brain
    Konishi Y, Stegmuller J, Matsuda T, Bonni S, Bonni A
    Science 303 1026-1030 2004 Refereed
  • A CAMKII-NeuroD signaling pathway regulates dendritic morphogenesis
    Gaudilliere B, Konishi Y, de la Iglesia N, Yao G, Bonni A
    Neuron 41 229-241 2004 Refereed
  • Characterization of a neurotrophin signaling mechanism that mediates neuron survival in a temporally specific pattern
    Shalizi A, Lehtinen M, Gaudilliere B, Donovan N, Han J, Konishi Y, Bonni A
    J Neurosci. 23 7326-7336 2003 Refereed
  • The E2F-Cdc2 cell-cycle pathway specifically mediates activity deprivation-induced apoptosis of postmitotic neurons
    Konishi Y, Bonni A
    J Neurosci. 23 1649-1658 2003 Refereed
  • Seizure-mediated neuronal activation induces DREAM gene expression in the mouse brain
    Matsu-ura T, Konishi Y, Aoki T, Naranjo JR, Mikoshiba K, Tamura T
    Brain Res Mol Brain Res. 109 198-206 2002 Refereed
  • JNK phosphorylation and activation of BAD couples the stress-activated signaling pathway to the cell death machinery
    Donovan N, Becker EB, Konishi Y, Bonni A
    J Biol Chem. 277 40944-40949 2002 Refereed
  • Cdc2 phosphorylation of BAD links the cell cycle to the cell death machinery
    Konishi Y, Lehtinen M, Donovan N, Bonni A
    Mol Cell 9 1005-1016 2002 Refereed
  • Stimulation of gene expression of NeuroD-related factor in the mouse brain following pentylenetetrazol-induced seizures
    Konishi Y, Matsu-ura T, Mikoshiba K, Tamura T
    Brain Res Mol Brain Res. 97 129-136 2001 Refereed
  • Promoter structure and gene expression of the mouse inositol 1,4,5-trisphosphate receptor type 3 gene
    Tamura T, Hashimoto M, Aruga J, Konishi Y, Nakagawa M, Ohbayashi T, Shimada M, Mikoshiba K
    Gene. 275 169-176 2001 Refereed
  • Molecular cloning and characterization of neural activity-related RING finger protein (NARF): a new member of the RBCC family is a candidate for the partner of myosin V
    Ohkawa N, Kokura K, Matsu-Ura T, Obinata T, Konishi Y, Tamura T
    J Neurochem. 78 75-87 2001 Refereed
  • Identification of the C-terminal activation domain of the NeuroD-related factor (NDRF)
    Konishi Y, Aoki T, Ohkawa N, Matsu-Ura T, Mikoshiba K, Tamura T
    Nucleic Acids Res. 28 2406-2412 2000 Refereed
  • Distribution of AP-2 subtypes in the adult mouse brain
    Shimada M, Konishi Y, Ohkawa N, Ohtaka-Maruyama C, Hanaoka F, Makino Y, Tamura T
    Neurosci Res. 33 275-280 1999 Refereed
  • Transcriptional regulation of mouse type 1 inositol 1,4,5-trisphosphate receptor gene by NeuroD-related factor
    Konishi Y, Ohkawa N, Makino Y, Ohkubo H, Kageyama R, Furuichi T, Mikoshiba K, Tamura T
    J Neurochem. 72 1717-1724 1999 Refereed
  • Activation of the mouse inositol 1,4,5-trisphosphate receptor type 1 promoter by AP-2
    Ohkawa N, Konishi Y, Shimada M, Makino Y, Yoshikawa S, Mikoshiba K, Tamura T
    Gene 229 42327 1999 Refereed
  • Transcription initiation sites and promoter structure of the mouse type 2 inositol 1,4,5-trisphosphate receptor gene
    Morikawa K, Ohbayashi T, Nakagawa M, Konishi Y, Makino Y, Yamada M, Miyawaki A, Furuichi T, Mikoshiba K, Tamura T
    Gene 196 181-185 1997 Refereed
  • Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type 1 inositol 1,4,5-trisphosphate receptor gene
    Konishi Y, Kobayashi Y, Kishimoto T, Makino Y, Miyawaki A, Furuichi T, Okano H, Mikoshiba K, Tamura T
    J Neurochem. 69 476-484 1997 Refereed
  • Analysis of Glial-Specific Gene Expression Using in Vitro Transcription Assays
    Tamura T, Konishi Y, Makino Y, Mikoshiba K
    Methods 10 312-319 1996 Refereed
  • Inhibition of glycogen synthase kinase-3 reduces extension of the axonal leading process by destabilizing microtubules in cerebellar granule neurons
    Inami, Yoshihiro;Omura, Mitsuru;Kubota, Kenta;Konishi, Yoshiyuki
    BRAIN RESEARCH 1690 51-60 Jul.  2018
  • Kinesin-1 sorting in axons controls the differential retraction of arbor terminals.
    Seno Takeshi;Ikeno Tatsuki;Mennya Kousuke;Kurishita Masayuki;Sakae Narumi;Sato Makoto;Takada Hiroki;Konishi Yoshiyuki
    Journal of cell science 129(18) 2016
    :The ability of neurons to generate multiple arbor terminals from a single axon is crucial for establishing proper neuronal wiring. Although growth and retraction of arbor terminals are differentially regulated within the axon, the mechanisms by which neurons locally control their structure remain largely unknown. In the present study, we found that the kinesin-1 (Kif5 proteins) head domain (K5H) preferentially marks a subset of arbor terminals. Time-lapse imaging clarified that these arbor terminals were more stable than others, because of a low retraction rate. Local inhibition of kinesin-1 in the arbor terminal by chromophore-assisted light inactivation (CALI) enhanced the retraction rate. The microtubule turnover was locally regulated depending on the length from the branching point to the terminal end, but did not directly correlate with the presence of K5H. By contrast, F-actin signal values in arbor terminals correlated spatiotemporally with K5H, and inhibition of actin turnover prevented retraction. Results from the present study reveal a new system mediated by kinesin-1 sorting in axons that differentially controls stability of arbor terminals.
  • Study of local intracellular signals regulating axonal morphogenesis using a microfluidic device.
    Uryu Daiki;Tamaru Tomohiro;Suzuki Azusa;Sakai Rie;Konishi Yoshiyuki
    Science and technology of advanced materials 17(1) 2016
    :The establishment and maintenance of axonal patterning is crucial for neuronal function. To identify the molecular systems that operate locally to control axonal structure, it is important to manipulate molecular functions in restricted subcellular areas for a long period of time. Microfluidic devices can be powerful tools for such purposes. In this study, we demonstrate the application of a microfluidic device to clarify the function of local Ca(2+) signals in axons. Membrane depolarization significantly induced axonal branch-extension in cultured cerebellar granule neurons (CGNs). Local application of nifedipine using a polydimethylsiloxane (PDMS)-based microfluidic device demonstrated that Ca(2+) entry from the axonal region via L-type voltage-dependent calcium channels (L-VDCC) is required for branch extension. Furthermore, we developed a method for locally controlling protein levels by combining genetic techniques and use of a microfluidic culture system. A vector for enhanced green fluorescent protein (EGFP) fused to a destabilizing domain derived from E. coli dihydrofolate reductase (ecDHFR) is introduced in neurons by electroporation. By local application of the DHFR ligand, trimethoprim (TMP) using a microfluidic device, we were able to manipulate differentially the level of fusion protein between axons and somatodendrites. The present study revealed the effectiveness of microfluidic devices to address fundamental biological issues at subcellular levels, and the possibility of their development in combination with molecular techniques.
  • Inhibition of glycogen synthase kinase-3 reduces extension of the axonal leading process by destabilizing microtubules in cerebellar granule neurons.
    Inami Yoshihiro;Omura Mitsuru;Kubota Kenta;Konishi Yoshiyuki
    Brain research 1690 2018
    :Recent studies have uncovered various molecules that play key roles in neuronal morphogenesis. Nevertheless, the mechanisms underlying the neuron-type-dependent regulation of morphogenesis remain unknown. We have previously reported that inhibition of glycogen synthase kinase-3 (GSK3) markedly reduced axonal length of cerebellar granule neurons (CGNs) in a neuron-type-dependent manner. In the present study, we investigated the mechanisms by which the growth of CGN axons was severely suppressed upon GSK3 inhibition. Using time-lapse imaging of cultured CGNs at early morphogenesis, we found that extension of the leading process was severely inhibited by the pharmacological inhibition of GSK3. The rate of somal migration was also reduced with a GSK3 inhibitor in dissociated culture as well as in microexplant culture. In addition, CGNs ectopically expressed with a catalytically inactive mutant of GSK3 exhibited a migration defect in vivo. In axonal leading processes of CGNs, detyrosination and acetylation of α-tubulin, which are known to correlate with microtubule stability, were decreased by GSK3 inhibition. A photoconversion analysis found that inhibition of GSK3 increases the turnover of microtubules. Furthermore, in the presence of paclitaxel, a microtubule-stabilizing reagent, inhibition of GSK3 recovered the axonal leading process extension that was reduced by paclitaxel. Our results suggest that GSK3 supports the extension of axonal processes by stabilizing microtubules, contrary to its function in other neuron-types, lending mechanical insight into neuron-type-dependent morphological regulation.

Conference Activities & Talks

  • Intracellular molecular systems regulating the branched axonal structure
    Y Konishi
    Basic and Translational Research for Brain Disease Symposium Dec.  2018
  • Region dependent regulatory mechanisms for the maintenance of axonal arbor structure
    Y. Konishi, N. Matsumoto, I. Hori, C. Imanaka, Miyake
    12th International Symposium on Nanomedicine Dec.  2018
  • Distribution regularity of mitochondria and en passant presynaptic sites along axon in cerebellar granule neurons
    Ikuma Hori, Nozomu Matsumoto, Seiji Miyake, Yoshiyuki Konishi
    Neuroscience 2019 Oct.  2019
  • Distribution regularity of mitochondria and en passant presynaptic sites along axon in cerebellar granule neurons
    Ikuma Hori, Nozomu Matsumoto, Seiji Miyake, Yoshiyuki Konish
    16th Asian society for mitochondrial research and medicine Oct.  2019
  • Temporal and spatial regulation of stationary mitochondria in axon
    Y. Konishi, N. Matsumoto, I. Hori, S. Miyake
    11th International Symposium on Nanomedicine Dec.  2017
  • The cell-intrinsic molecular systems controlling the region specific stability of branched axons
    Yoshiyuki Konishi
    The 6th Taiwan-Japan Symposium on Nanomedicine Jan.  2015
  • Spatial cell signaling mediated by microtubule regulations contributes to the maintenance of neuronal morphology
    小西 慶幸
     Sep.  2012
  • Tubulin polyglutamylation regulates cytoskeletal distribution in brain
    Ohata K, Konishi Y, Tsuchiya R, Ikegami K, Setou M
     Mar.  2012
  • Local regulation of axonal transport and its possible contribution to the axonal branch pattering
     Mar.  2012
  • The Role of Plyglutamylation on the Regulation of Neuronal Cytoskeleton
    Ohata K, Konishi Y, Tsuchiya R, Ikegami K, Setou M
     Dec.  2011
  • The regulation and function of post-translational modification of tubulins in neurons
    Konishi Y, Ohata K, Tsuchiya R, Ikegami K, Setou M
     Aug.  2011
  • The region specific control of branch stability in axonal arbors mediated by kinesin mediated transport
    Konishi Y, Seno T, Kubota K, Sakae N, Takada H
    9th International Symposium on Nanomedicine Dec.  2015
  • Local regulation of molecular reactions in maintaining axonal integrity
    Yoshiyuki Konishi, Takeshi Seno, Tatsuki Ikeno, Kousuke Mennya, Masayuki Kurishita, Narumi Sakae, Hiroki Takada
    3nd International Symposium on Nanomedicine Molecular Science3nd International Symposium on Nanomedicine Molecular Science Nov.  2015

Awards & Honors

  •  The Award for Distinguished Young Investigator of Japanese Society for Neurochemistry2010
  •  Harvard School of Public Health, Taplin fellowship2000